Abstract
The terminal segment of the Bacillus FTU respiratory chain has been studied. The shape of difference spectra and biphasic cyanide titration curve point to the presence of two terminal oxidases ( aa 3- and o-type) in the subcellular membrane vesicles of this Bacillus. The aa 3-type oxidase was shown to dominate in the beginning of the logarithmic phase while the o-type oxidase was more pronounced at the stationary phase of bacterial growth. Both enzymes oxidized TMPD in the presence of ascorbate: the o-type oxidase with K m = 0.7 mM TMPD and V max = 2.2 μmol O 2 min −1 nmol −1 haem o and the aa 3-type oxidase with K m = 2 mM TMPD and V max = 11.2 μmol O 2 min −1 nmol −1 haem a. KCN inhibited the two oxidases (half-maximal inhibition at 2 μM and 20 μM for the aa 3- and o-type oxidases, respectively).
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