Abstract
As a primary bacterial pathogen in companion animals, Staphylococcus pseudintermedius has zoonotic potential. This pathogen exhibits multidrug resistance, including resistance to methicillin, and biofilm-forming ability, making it hard to eradicate with antimicrobial agents. One potential alternative is bacteriophage therapy. In this study, we first characterized the antimicrobial resistance profile of S. pseudintermedius from canine samples and isolated two novel bacteriophages, pSp-J and pSp-S, from canine pet parks in South Korea to potentially control S. pseudintermedius. The biological characteristics of phages were assessed, and the phages could infect most of the methicillin-resistant S. pseudintermedius strains. We found that these phages were stable under the typical environment of the body (~37°C, pH 7). We also assessed bacterial lysis kinetics using the two phages and their cocktail, and found that the phages could prevent biofilm formation at low doses and could degrade biofilm at high doses. Taken together, this study demonstrates that bacteriophages pSp-J and pSp-S isolated in this study can be used to potentially treat methicillin-resistant S. pseudintermedius.
Highlights
Staphylococcus pseudintermedius is an opportunistic pathogen commonly isolated from dogs and other companion animals
We characterized these phages and assessed their stability in different environmental conditions. We determined their anti-bacterial and anti-biofilm activities. We found that these phages, pSp-J and pSp-S, can prevent biofilm formation at low doses and degrade biofilm at high doses, suggesting that these phages may potentially be used for treatment of methicillin-resistant S. pseudintermedius (MRSP) infection
Dog to human transmission and implantable material related surgical site infection of S. pseudintermedius has been reported in human cases [2, 40, 41]
Summary
Staphylococcus pseudintermedius is an opportunistic pathogen commonly isolated from dogs and other companion animals. Given the recent rise in pet ownership, the number of reported cases of S. pseudintermedius infection in humans has risen as well [1, 2]. We isolated two novel phages, pSp-J and pSp-S, from soil and water samples from pet parks in South Korea for possible application in controlling MRSP. We characterized these phages and assessed their stability in different environmental conditions. We determined their anti-bacterial and anti-biofilm activities We found that these phages, pSp-J and pSp-S, can prevent biofilm formation at low doses and degrade biofilm at high doses, suggesting that these phages may potentially be used for treatment of MRSP infection
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