Abstract
A procedure for the quantitative extraction of tocopherol derivatives from plasma was systematically developed using an efficient liquid—liquid distribution process. An aqueous stationary phase was coated on silica gel or diatomaceous earth powder in a closed-bed glass column prepared by slurry packing. A liquid—liquid chromatographic system provided with a detector was introduced as a preliminary extraction process. Liquid—solid chromatography followed as the second dimension of the separation and was used to determine α-tocopherol nicotinate in the extract. Stationary and mobile phases in the two-dimensional liquid chromatographic system were optimized to determine tocopherol derivatives. A highly sensitive quantitation method for α-tocopherol nicotinate in plasma was developed by using liquid—liquid and liquid—solid chromatography in conjunction with column switching by a tube-type micro-evaporator.
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