Abstract
Identification of target molecules of new bioactive compounds is still a challenge in drug development. Various proteomics-based methods have been developed to analyze the interaction between compounds and target proteins. Among these methods, cellular thermal shift assay (CETSA) has been frequently applied in recent years for validation studies of compound-protein interactions using antibodies. Combining CETSA with comprehensive proteomic analysis has been successful in narrowing down the target(s) of a new compound from the enormous number of proteins in cell. In this chapter, we introduce 2DE-CETSA, which combines CETSA with proteome analysis using two-dimensional electrophoresis as a method for identification of target proteins.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
