Abstract
Background In high-cell density fermentations the host cells are often subjects of transient changes in microenvironment around them. This is true especially in large-scale bioreactors. The changes can be for example substrate gradient, differences in oxygen availability and pH variations. Our aim is to obtain more information about physiological changes of E.coli W3110 and its recombinant variants in such conditions for better understanding of the bottlenecks in recombinant protein production processes.
Highlights
In high-cell density fermentations the host cells are often subjects of transient changes in microenvironment around them
The 4th Recombinant Protein Production Meeting: a comparative view on host physiology The organisers would like to thank Novozymes Delta Ltd who generously supported the meeting. Meeting abstracts – A single PDF containing all abstracts in this supplement is available here. http://www.biomedcentral.com/content/pdf/1475-2859-5-S1-info.pdf
To mimic the conditions in large-scale fermentations, we have set-up a two-compartment bioreactor [1], in which cells are circulated between a regular stirred tank reactor (STR) and a plug-flow reactor (PFR) using a peristaltic pump
Summary
In high-cell density fermentations the host cells are often subjects of transient changes in microenvironment around them. Two-compartment bioreactor as a scale-down model to study the effect of glucose overflow and anaerobiosis on large-scale recombinant protein production processes * Corresponding author from The 4th Recombinant Protein Production Meeting: a comparative view on host physiology Barcelona, Spain. Published: 10 October 2006 Microbial Cell Factories 2006, 5(Suppl 1):P26 doi:10.1186/1475-2859-5-S1-P26
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