Abstract
This paper puts forward a kind of two benches structure of the glass-PDMS free flow electrophoresis chip. We use die casting method to produce microstructure, and use PDMS as its structure layer. The mold is made in tape-PMMA by micro cementing technology. During the progress of PDMS curing, gold wires are directly integrated in the electrode groove to form the chips electrodes. PDMS and glass are sealed in natural bonding method. Chips glass surface of separation chamber realizes the modification through sticking polyimide tape. Visual technology is conducted for chip electrophoresis separation. The sample is the mixture of methyl green and Rhodamine B. The separation effect of methyl green and Rhodamine B is obvious when the electric field is 31 V/cm. the "two benches" between the electrode groove and the separation chamber block the electrolytic bubbles into the separation chamber. When the separation chamber height is 70 μm and the electric field is 50 V/cm, the air bubbles can be glidingly discharged from electrode groove. The samples steady separation time is more than 2 hours, and this free flow electrophoresis chip realizes continuous work.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
