Two anatomical pathways for the renewal of surface glycoproteins in chloride cells of fish gills.

Abstract

The cytoplasm of chloride cells found in the epithelium lining the gills of guppies (Lebistes reticulatus) contains, in addition to the Golgi apparatus and cisternae of endoplasmic reticulum, two distinct membranous components, the vesiculotubular and the tubular systems. While the latter is connected to the laterobasal plasma membrane, the former, made up of small vesicles and short membranous tubules, is seen mainly between the Golgi apparatus and the apical cavity which invaginates the apex of the cell. The role of these two systems in the transport of glycoproteins from the Golgi apparatus to the cell surface was investigated in fishes maintained in fresh and salt water, injected with 3H-fucose, and sacrificed at various intervals thereafter (10 and 30 min; 2.5, 8, 15.5, 24, and 48 hours). The distribution of the label was analyzed by quantitative radioautography in sections examined with the light and electron microscopes. The light microscopic data suggested that the label incorporated in the supranuclear region, where the Golgi apparatus is located, migrated toward the apical and the laterobasal regions of the chloride cells. The relative concentration of the tracer over the various components of the cytoplasm of these cells was calculated from data collected on electron microscope radioautographs at various intervals after 3H-fucose injection. The curves obtained supported the view that glycoproteins synthesized in the Golgi apparatus were transported to the apical surface via the vesiculotubular system, and to the laterobasal membrane via the tubular system.