Twenty-two years later: consistent dose estimation of an accidental overexposure by retrospective biological dosimetry.

Abstract

The goal of this study was to retrospectively estimate the exposure dose of a victim from the Lilo radiological accident in Georgia after 22 y and compare it with the original cytogenetics-based analysis performed in our laboratory. Similar types of studies have been published, notably involving victims of the Chernobyl, Goiânia and Tammiku accidents. Nevertheless, their estimations were done after shorter periods of time post-exposure, and in some cases, the exposure might not have been exclusively of an external nature. In this study, Fluorescence In Situ Hybridization (FISH) was used to score chromosomal translocations in lymphocytes from a recent blood sample of the victim and the dose assessment was performed using our laboratory's FISH calibration curve. The resulting whole-body exposure dose of 0.35Gy [0.12, 0.72] was similar to the one obtained when reanalysing the original scoring data from 1997 (0.38Gy [0.13-0.75]), suggesting that our current calibration curve could be used for relative dose estimations long time after external exposure.