Abstract
It has been proved nanomaterials can improve the efficiency of biogas production in dark fermentation. However, the specific relationship between anaerobic bacteria and surface charge on nanomaterials has not been clear. In this research, ZnMoO4 with variable surface charges are prepared to study their interaction during anaerobic hydrogen-producing. A series of experiments and dynamic analysis indicate that ZnMoO4 with an isoelectric point at 8.8 has the highest H2 yield of 296.3 mL/g straws, which is 140.7 % higher than the control group. The production of acetic acid and butyric acid from metabolism has a significant increase along with the improvement of total metabolites, a much higher performance than individual Zn2+ and MoO42-. Moreover, the abundance of Clostridium sensus stricta 10 has an obvious enhancement in the dominant microbial communities at 55℃, promoting the change of metabolic pathway with high stability and low cytotoxicity. These results provide meaningful references to a higher H2 yield along with optimum production processes.
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