Abstract
Today most cardiovascular surgeons prefer artery grafts to vein grafts, because of their better long term patency. Recently we reported monocyte/macrophage infiltration and the development of intimal hyperplasia (IH) is different in rat epigastric vein to common femoral artery interposition graft, from common femoral artery transection and re-anastomosis. Tumor Necrosis Factor-α (TNF-α) has been well known as one of the growth factors produced from activated macrophages. We hypothesize TNF-α mRNA expression is different in vein graft compared to femoral artery re-anastomosis. Lewis rats underwent either epigastric vein to common femoral artery interposition grafts (VG: n=25) or common femoral artery transection and re-anastomoses (FA: n=24). Vessels were harvested at 0 hour, 1 hour, 3 hours, 1 day, 4 days, 1 week and 2 weeks. To compare the development of IH, vessel wall areas were calculated and described as the intimal area/total area (Ri). TNF-α mRNA expression was examined by using reverse transcription-polymerase chain reaction, and was compared with S-26 mRNA expression on computerized densitometry. The TNF-α/S-26 mRNA expression ratio was increased in VG compared to FA re-anastomosis, especially at 1 week (p<0.01). These results suggest that the mechanism of IH is different between vein graft and artery graft. Japanese patients [1–4]. The majority of bypass failures occur within the first two years, due to the process of intimal hyperplasia (IH) which begins as early as one month after operation [5]. Recently we reported monocyte/macrophage infiltration, and the development of IH is different in rat epigastric vein to common femoral artery interposition graft (VG), from common femoral artery transection & reanastomosis (FA) [6]. Tumor Necrosis Factor-α (TNF-α) has been well known as one of the growth factors produced from activated macrophages [7]. To study the mechanism of IH, we hypothesize TNF-α mRNA expression is different in VG from FA re-anastomosis.
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