Tumor Antigen Presenting Cells (TAPCells) induce homing receptor expression on T lymphocytes associated with migration to tumor sites

Abstract

Event Abstract Back to Event Tumor Antigen Presenting Cells (TAPCells) induce homing receptor expression on T lymphocytes associated with migration to tumor sites Ignacio P. Avalos Carrasco1, 2, María A. Gleisner1, 2, Cristian Pereda1, 2, Mercedes López1, 2, Fermin González1, 2 and Flavio A. Salazar-Onfray1, 2* 1 Universidad de Chile, Institute of Biomedical Sciences, Chile 2 Millennium Institute on Immunology and Immunotherapy, Chile Introduction: Dendritic cells (DC) play an essential role in the induction of adaptive immunity. We have previously generated antigen presenting cells stimulated with an allogeneic melanoma-derived cell lysate named as Tumor Antigen Presenting Cells (TAPCells). In melanoma patients, TAPCells injection induces T cell-mediated responses in 60% of patients, establishing positive correlation between anti-tumor immune response and patient survival. Despite these promising results, it is unknown whether TAPCells are able to imprint in T cells a homing phenotype that allows them to enter tumor sites. Given that appropriate T cell migration is essential for the generation of an efficient antitumor response, we investigated whether TAPCells trigger the expression of homing receptors associated to tumor sites. Material and Methods: First, using flow cytometry, we measured the expression of activation markers, cell adhesion molecules and chemokine receptors CCR4, CXCR3 and CXCR4 on T cells activated with TAPCells. Secondly, we monitored the functionality of these receptors through transmigration assays in vitro. Results: Coculture of T cells with TAPCells significantly increased the expression of CXCR3 and CXCR4 together with activation markers on CD4+ T cells, and the expression of CCR4 and CXCR3 on CD8+ T cells compared with non-stimulated lymphocytes. In addition, TAPCells-stimulated T cells showed higher expression of the adhesion molecule CD11a in both T cells populations in comparison with non-activated T cells. Finally, migration assays showed that TAPCells-stimulated T cells have improved mobilization capacity than control cells and display an increased migration in presence of ligands for CXCR3 and CXCR4. Discussion: Our results show that TAPCells are capable of inducing homing receptors on T cells with the potential to direct them into inflamed skin and tumor tissues, proving their capacity to modulate in vitro migration of these cells. As a projection of this work, we will corroborate these results in an in vivo murine model. These findings contribute to a better understanding of the role of TAPCells on T cell stimulation and migration and gives prospects for the improvement of TAPCells-based immunotherapy. Acknowledgements Funded by grants FONDEF-D11I1036; FONDECYT-1130320; FONDECYT-1130324; IMII P09/016-F. Keywords: Melanoma cancer, TAPCells, Immunotherapy, Homing, Chemokines Receptors Conference: IMMUNOCOLOMBIA2015 - 11th Congress of the Latin American Association of Immunology - 10o. Congreso de la Asociación Colombiana de Alergia, Asma e Inmunología, Medellin, Colombia, 13 Oct - 16 Oct, 2015. Presentation Type: Poster Presentation Topic: Immunotherapy Citation: Avalos Carrasco IP, Gleisner MA, Pereda C, López M, González F and Salazar-Onfray FA (2015). Tumor Antigen Presenting Cells (TAPCells) induce homing receptor expression on T lymphocytes associated with migration to tumor sites. Front. Immunol. Conference Abstract: IMMUNOCOLOMBIA2015 - 11th Congress of the Latin American Association of Immunology - 10o. Congreso de la Asociación Colombiana de Alergia, Asma e Inmunología. doi: 10.3389/conf.fimmu.2015.05.00082 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 15 May 2015; Published Online: 14 Sep 2015. * Correspondence: PhD. Flavio A Salazar-Onfray, Universidad de Chile, Institute of Biomedical Sciences, Santiago, Chile, fsalazar@u.uchile.cl Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Ignacio P Avalos Carrasco María A Gleisner Cristian Pereda Mercedes López Fermin González Flavio A Salazar-Onfray Google Ignacio P Avalos Carrasco María A Gleisner Cristian Pereda Mercedes López Fermin González Flavio A Salazar-Onfray Google Scholar Ignacio P Avalos Carrasco María A Gleisner Cristian Pereda Mercedes López Fermin González Flavio A Salazar-Onfray PubMed Ignacio P Avalos Carrasco María A Gleisner Cristian Pereda Mercedes López Fermin González Flavio A Salazar-Onfray Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.