Abstract
Several kinetoplastid mitochondrial genes cannot be expressed without RNA editing of their transcripts to create a functional open reading frame. We have examined one such mitochondrial gene, CR4, in Trypanosoma brucei EATRO164 and find extensive editing of transcripts in both bloodstream and procyclic life cycle stages. However, a consensus edited sequence for the entire mRNA occurs only in the bloodstream stage. The unedited CR4 transcript is 283 nucleotides in length, not including the poly(A) tail. A total of 325 uridines are inserted, and 40 uridines deleted, to create the mature mRNA which encodes a very hydrophobic protein.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
