Abstract
The aim of this study was to identify using molecular methods the filamentous fungi associated with the Golot cheese produced in Ardesen, Rize Province, East Black Sea region, Turkey. Sixteen fungi were isolated from seven cheese samples and subjected to sequencing analysis using different fungal DNA barcodes. The first marker used, internal transcribed spacer (ITS), allowed identification of Geotrichum candidum and Penicillium roqueforti. The other isolates, defined as Penicillium and Cladosporium, could be identified only at the genus level using ITS; therefore, the beta-tubulin (benA) gene and the microsatellite PC4 markers were used to define the remaining Penicillium isolates as P. biforme and P. solitum. The Cladosporium isolate was identified as C. macrocarpum by analysis using benA and translation elongation factor 1-α (tef1) genes. The most common species associated with Golot cheeses were determined to be P. biforme (56%) and G. candidum (25%). Previously unknown sequences of P. solitum PC4 and C. macrocarpum tef1, and a longer version of C. macrocarpum benA were submitted to GenBank.
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