Abstract
Cultures of Blakeslea trispora in which (+) and (−) mycelia were separated by membrane filters for 152 h made 4.5, 13.3, and 25.5% of the trisporic acids (102 μg/ml of medium) made by combined (+) and (−) cultures. Control studies indicate that the extent of trisporic acid biosynthesis in cultures with membrane-separated mycelia is proportional to the rate of diffusion of small molecules through the membrane.
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