Trichotorquatus salinus sp. nov. (Oculatellaceae, Cyanobacteria) from a Saltern of Gomso, Republic of Korea

Abstract

Six strains of subaerial cyanobacteria were isolated from a Gomso saltern in the Republic of Korea, all of which were studied using morphological and molecular traits. Trichotorquatus salinus sp. nov. was studied using a light microscope (LM), transmission electron microscope (TEM), 16S rRNA, 16S–23S ITS region, and ecological data. T. salinus is a thin and simple filament with a false branch and a firm collar sheath. The phylogenetic analyses of 16S rRNA revealed that Trichotorquatus formed a monophyletic lineage and the strains of T. salinus formed a distinct clade among the species in the genus Trichotorquatus. In the statistical analysis, the inter-species genetic distance of the five species of Trichotorquatus, including T. salinus, is shown to be greater than the distance of the previously reported species of Trichotorquatus. Additionally, 16S–23S ITS gene sequences between T. salinus and four species of Trichotorquatus showed dissimilarities of 55.3–59.4%. In the secondary structure of 16S–23S ITS region (type 2 operon), D1–D1′, Box-B, and V3 helix of T. salinus were different from the other taxa in the genus Trichotorquatus. These results demonstrate that T. salinus sp. nov. has unique morphological, ecological, and molecular traits. Therefore, we propose that T. salinus sp. nov. is a novel species belonging to the genus Trichotorquatus.