Abstract
The present study investigated the synergistic effects of a novel combined uncoupler of TCS and TCP on excess activated sludge reduction during a 60-day operation using a sequence batch reactor (SBR). Response surface methodology (RSM) was employed to obtain the optimal dosage of the combined uncoupler. The results of 60-day operation demonstrated the combined uncoupler had effectively reduced the sludge yield by approximately 52%, without serious affecting the substrate removal efficiency. The high sludge reduction rate revealed that it was feasible and effective to utilize a combined uncoupler to limit excess activated sludge. The three-dimensional excitation-emission matrix (EEM) fluorescence spectroscopy analysis of activated sludge with different metabolic uncouplers indicated that tryptophan, tyrosine protein-like substances and tryptophan, tyrosine amino-like substances were reduced by adding a combined uncoupler. Moreover, the variation of sludge components provided a better understanding of the effects of uncouplers on activated sludge reduction.
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