Abstract

The purpose of this work was to determine whether sequences homologous to maize transposable elements exist in wheat and its relatives. Genomic Southern blots of Triticeae members were used to detect copy number of homologues to the major open reading frames of the maize Ac and En transposases, and MudrB, a regulator of transposition. The number of similar sequences was the least with Ac, intermediate with Mu, and the greatest with En. Maize transposons at the nucleotide and amino acid level were used in EST and genomic database searches. Nucleotide identity between maize and wheat was 58% for Ac, 59% for Mu, and 61% for En. Protein homologues were found for Ac and Mu in wheat and barley, and for En in barley.

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