Abstract
Jasmonates (jasmonic acid and its relatives) are a group of oxylipin phytohormones that are implicated in the regulation of a range of developmental processes and responses to environmental stimuli in plants. The biosynthesis of JAs occur sequentially in various subcellular compartments including the chloroplasts, peroxisomes and the cytoplasm. The biologically active jasmonoyl-isoleucine (JA-Ile) activates the core JA signaling in the nucleus by binding with its coreceptor, SCFCOI1-JAZ. Five members of a clade of ATP-binding cassette G (ABCG) transporters of Arabidopsis thaliana were identified as the candidates of jasmonate transporters (JATs) in yeast cells. Among these JATs, AtJAT1/AtABCG16, has a dual localization in the plasma membrane and nuclear envelop and mediates the efflux of jasmonic acid (JA) across the plasma membrane and influx of JA-Ile into the nucleus. Genetic, cellular and biochemical analyses have demonstrated that AtJAT1/AtABCG16 is crucial for modulating JA-Ile concentration in the nucleus to orchestrate JA signaling. AtJAT1 could also be involved in modulating the biosynthesis of JA-Ile by regulating the distribution of JA and JA-Ile in the cytoplasm and nucleus, which would contribute to the highly dynamic JA signaling. Furthermore, other JAT members are localized in the plasma membrane and possibly in peroxisomes. Characterization of these JATs will provide further insights into a crucial role of transporter-mediated subcellular distribution in the metabolism and signaling of plant hormones, an emerging theme supported by the identification of increasing number of endomembrane-localized transporters.
Highlights
Reviewed by: Joelle Sasse Schlaepfer, Lawrence Berkeley National Laboratory, United States Department of Energy (DOE), United States
Five members of a clade of ATP-binding cassette G (ABCG) transporters of Arabidopsis thaliana were identified as the candidates of jasmonate transporters (JATs) in yeast cells
Cellular and biochemical analyses have demonstrated that AtJAT1/AtABCG16 is crucial for modulating jasmonic acid (JA)-Ile concentration in the nucleus to orchestrate JA signaling
Summary
Five members of a clade of ATP-binding cassette G (ABCG) transporters of Arabidopsis thaliana were identified as the candidates of jasmonate transporters (JATs) in yeast cells Among these JATs, AtJAT1/AtABCG16, has a dual localization in the plasma membrane and nuclear envelop and mediates the efflux of jasmonic acid (JA) across the plasma membrane and influx of JA-Ile into the nucleus. The degradation of JAZ proteins relieves their repression on the activity of the key transcription factors (e.g., MYC2), activating the core JA signaling pathway (Chini et al, 2007; Thines et al, 2007; Browse, 2009; Sheard et al, 2010) Both the COI1 and JAZ proteins are localized in the nucleus and their nuclear localization was not altered in the mutants of the other interacting partner (Chini et al, 2007; Withers et al, 2012).
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
