Abstract
Objective To investigate the survival and differentiation of enhanced green fluorescent protein (EGFP)-labeled neurotrophin-3 (NT-3) modified fetal spinal cord-derived neural stem cells (NSCs) after transplanted into injured spinal cord.Methods EGFP labeled NT-3 modified NSCs were transplanted into the lesioned site of T9 hemisected rats (n = 10).The survival of grafted cells, expressions of NT-3 and differentiation into oligodendrocytes were detected by immunohistochemical assessment, and compared with the rats in unmodified NSCs transplantation group (n = 10).Results Cell counting presented more surviving cells in NT-3-NSCs group (304 ± 40) than NSCs group (258 ± 41) (P 0.05).The percentage of NT-3 positive cells in NT-3-NSCs group [(74.2 ± 14.1)%] was higher than NSCs group [(22.9 ± 11.1) %] (P < 0.01).More grafted cells in NT-3-NSCs group [(53.8 ± 12.4) %] differentiated into oligedendrocytes than NSCs group [(39.7 ± 13.7) %] (P 0.05).Conclusion NT-3 modification improves the survival of grafted NSCs, and promotes the expression of NT-3 and the differentiation into oligodendrocytes, which can increase the therapeutic effect of NSCs transplantation for spinal cord injury. Key words: Spinal cord injury; Transplantation; Neural stem cells; Neurotrophin3
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