Abstract
Investigating the intracellular dynamics response to the spatio-temporal environmental stimuli to which cells are exposed can provide insights into signal transduction pathways and regulation mechanisms of cellular functions. Microfluidics has become an effective platform for precise control of the cellular microenvironment, and for high-throughput, automatic analysis of cellular dynamic process. In this study, a novel microfluidic-based system has been designed, which includes a peripheral fluid driving system and a microfluidic chip with a variable cross-section channel. Based on the microfluidic channel, the transmission characteristics of dynamic biochemical signals in both steady and pulsatile flows are systematically investigated through fluorescence signal experiment and 3D COMSOL simulation. We conclude that the microfluidicbased system can effectively load dynamic biochemical and/or biomechanical signals on cells cultured within the microfluidic chamber, and that the transmission characteristics of dynamic biochemical signals are greatly influenced by biochemical signal frequency, pulsatile flow frequency and transmission distance. These influence factors should be considered for improving the loading of dynamic biochemical signals on cells for further cell analysis.
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