Abstract
High-molecular-weight RNA was isolated from BAI strain A avian leukosis (myeloblastosis) virus by the sodium dodecyl sulfate dithiothreitol technique. Three of five preparations induced infection in synchronized chick embryo cells (CEC) in tissue cultures pretreated with diethylaminoethyl-dextran. In 2 series of studies, virus particles released by the CEC into the culture fluids caused neoplasms typical of the action of BAI strain A virus manifested by myeloblastosis and tumors of the kidney, liver, and ovary when inoculated into newly hatched chickens. Some of the cultures exhibited pronounced CEC morphologic alteration. Virus produced in the altered CEC cultures was infectious for, and caused morphologic changes on, serial passage in fresh CEC, but did not infect or alter bone marrow cells. A third RNA preparation infected CEC but did not alter the cells, though the virus induced tumors in the chickens. Agent liberated by the CEC cultures interfered with Rous sarcoma virus and MC29 virus superinfection and activated RSV nonproducer cells. RNA fractions inducing infection contained no virus particles that could be seen in the electron microscope, and infectivity was abolished by ribonuclease treatment. Protein content of the RNA preparations was less than 6% which was 406-fold less than that in intact virus particles as demonstrated by use of double labeling.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
