Abstract
Transient gene expression has become a valuable tool to evaluate conditions for optimal gene transfer and to study the regulation of gene expression. When compared, the results obtained transiently parallel those seen in stable transformants. Some examples of transient gene expression studies using biolistic devices are shown in Table 1. In this chapter, we will describe transient expression in maize BMS cells, a well-established system for transient assays. We will then focus on the requirements for establishing a regulated transient gene expression system, using the maize seed aleurone expression system as a model.
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