Transient appearance of and regional differences in apical cell surface materials during early morphogenesis of the chicken lens

Abstract

Apical cell surface materials were analysed with staining and lectin histochemistry in the chicken lens, from the earliest stages of lens morphogenesis through the completion of primary fibre cell elongation. Acidic materials were found to accumulate on the apical cell surface of the presumptive lens fibres from the mid cup stage through the early stages of lens vesicle formation, peaking just before lens fibre cell elongation. These materials labelled strongly with concanavalin A, but not with soybean lectin. By the completion of fibre cell elongation, these materials were gone. Conversely, the apical surface of the future lens epithelial cells demonstrated neutral materials, which were also largely removed by the completion of primary fibre cell elongation. These materials labelled with both concanavalin A and soybean lectin. The identity of these materials is not known, but their location prior to and during chicken lens morphogenesis suggests that they may be involved in establishing polarity during elongation of the primary lens fibre cells.