Abstract
Transglutaminases (TGases) are a widely distributed family of proteins found in many tissues and body fluids of vertebrates. To date the following types have been distinguished: secretory, tissue, epidermal, keratinocyte, and hemocyte TGase as well as factor XIIIa and erythrocyte hand 4.2 TGases are difficult to isolate, as they tend to form irreversible aggregates under native conditions. In this review, the isolation procedures for the different types of TGases are summarized. The most common chromatographic separation methods used for TGase purification are size-exclusion and ion-exchange chromatography. Additionally, other chromatographic methods (hydrophobic-interaction, affinity, adsorption chromatography) and electrophoretic techniques [preparative isoelectric focusing, sodium dodecyl sulphate polyacrylamide gel electrophoresis and zone electrophoresis] are described. Based on the enzymatic function of TGases (cross-linking of a primary amine and peptide bound glutamine), several established activity assays are described.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Journal of chromatography. B, Biomedical applications
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
