Abstract
SUMMARY: Reversions to wild type occurred when conidia of a number of biochemically deficient mutants of Aspergillus niger were treated with DNA from the wild-type strain. No reversions or wild-type colonies were obtained when an equivalent number of conidia from deficient strains were either treated with the same DNA as the recipient, or were plated without any DNA treatment. Increase in the percentage of transformation was observed up to 6 μg./ml. of donor DNA. The transforming activity of the donor DNA was found to be inhibited by the action of u.v. radiation, heat and DNase. The frequency of transformation was low which could be attributed to the method of extraction of DNA which involved crushing of cells in the presence of alumina, possibly breaking the DNA into small fragments thus making it biologically less active.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
