Abstract
ABSTRACTObjective: Transferability of significantly associated loci or GWAS “hits” adds credibility to genotype-disease associations and provides evidence for generalizability across different ancestral populations. We sought evidence of association of known asthma-associated single nucleotide polymorphisms (SNPs) in an African American population. Methods: Subjects comprised 661 participants (261 asthma cases and 400 controls) from the Howard University Family Study. Forty-eight SNPs previously reported to be associated with asthma by GWAS were selected for testing. We adopted a combined strategy by first adopting an “exact” approach where we looked-up only the reported index SNP. For those index SNPs missing form our dataset, we used a “local” approach that examined all the regional SNPs in LD with the index SNP. Results: Out of the 48 SNPs, our cohort had genotype data available for 27, which were examined for exact replication. Of these, two SNPs were found positively associated with asthma. These included: rs10508372 (OR = 1.567 [95%CI, 1.133-2.167], P = 0.0066) and rs2378383 (OR = 2.147 [95%CI, 1.149–4.013], P = 0.0166), located on chromosomal bands 10p14 and 9q21.31, respectively. Local replication of the remaining 21 loci showed association at two chromosomal loci (9p24.1-rs2381413 and 6p21.32-rs3132947; Bonferroni-corrected P values: 0.0033 and 0.0197, respectively). Of note, multiple SNPs in LD with rs2381413 located upstream of IL33 were significantly associated with asthma. Conclusions: This study has successfully transferred four reported asthma-associated loci in an independent African American population. Identification of several asthma-associated SNPs in the upstream of the IL33, a gene previously implicated in allergic inflammation of asthmatic airway, supports the generalizability of this finding.
Highlights
With an estimated 25.7 million people having asthma in 2010 and an increase of its prevalence from 7.3% in 2001 to 8.4% in 2010 [1], asthma has reached epidemic proportions in the United States
Multiple single nucleotide polymorphisms (SNPs) in linkage disequilibrium (LD) with rs2381413 located upstream of IL33 were significantly associated with asthma
Identification of several asthma-associated SNPs in the upstream of the IL33, a gene previously implicated in allergic inflammation of asthmatic airway, supports the generalizability of this finding
Summary
With an estimated 25.7 million people having asthma in 2010 and an increase of its prevalence from 7.3% in 2001 to 8.4% in 2010 [1], asthma has reached epidemic proportions in the United States. Current asthma prevalence is higher among non-Hispanic Blacks (9.5%) compared to their White counterparts (7.8%) [2]. Overall estimates for heritability of asthma and related traits suggest that 36–75% of asthma risk is attributable to genetic factors [5]. Genetic role in asthma predisposition is suggested by twin, linkage, and association studies [6,7]. The advent and application of genome-wide association studies (GWAS) has greatly improved our understanding of the genetic factors responsible for asthma. More than 20 broadly defined major susceptibility regions for asthma and related traits have been identified by GWAS [6,7] of which at least 9 loci have been replicated in independent populations
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