Transfer RNA reduces the formation of primer artifacts during quantitative PCR.

Abstract

BioTechniquesVol. 27, No. 1 BenchmarksOpen AccessTransfer RNA Reduces the Formation of Primer Artifacts During Quantitative PCRStephen R. StürzenbaumStephen R. Stürzenbaum*Address correspondence to Dr. Stephen R. Stürzenbaum, University of Wales, Cardiff, School of Biosciences, Biomedical Building, Cardiff, Wales, UK. Internet: E-mail Address: sturzenbaumsr@cardiff.ac.ukUniversity of Wales, Cardiff, Wales, UKSearch for more papers by this authorPublished Online:22 Aug 2018https://doi.org/10.2144/99271bm08AboutSectionsPDF/EPUB ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinkedInRedditEmail "Transfer RNA Reduces the Formation of Primer Artifacts During Quantitative PCR." , 27(1), pp. 50–52FiguresReferencesRelatedDetailsCited ByThe identification and semi-quantitative assessment of gastrointestinal nematodes in faecal samples using multiplex real-time PCR assays9 August 2021 | Parasites & Vectors, Vol. 14, No. 1Reporting the limits of detection and quantification for environmental DNA assays16 September 2019 | Environmental DNA, Vol. 2, No. 3Effect of egg turning and incubation time on carbonic anhydrase gene expression in the blastoderm of the Japanese quail ( Coturnix c. japonica )British Poultry Science, Vol. 49, No. 5Deoxynucleotide Triphosphates and Buffer ComponentsUse of a PNA probe to block DNA-mediated PCR product formation in prokaryotic RT-PCRMikkel Bender, William E. Holben, Søren J. Sørensen & Carsten S. Jacobsen16 May 2018 | BioTechniques, Vol. 42, No. 5Analysis of strawberry genes differentially expressed in response to Colletotrichum infectionPhysiologia Plantarum, Vol. 128, No. 4Factors Affecting Quantitative PCR Assay of Plesiomonas shigelloidesFood Biotechnology, Vol. 20, No. 2Molecular cloning and characterization of Cpn60 in the free-living nematode Plectus acuminatusCell Stress & Chaperones, Vol. 10, No. 2The Application of Real-Time PCR to Food and Agricultural Systems. A Review6 February 2007 | Food Biotechnology, Vol. 18, No. 1LightCycler qPCR optimisation for low copy number target DNAJournal of Immunological Methods, Vol. 270, No. 1Comparison of Different Approaches To Quantify Staphylococcus aureus Cells by Real-Time Quantitative PCR and Application of This Technique for Examination of CheeseApplied and Environmental Microbiology, Vol. 67, No. 7A multiplex PCR assay for differentiating economically important gastrointestinal nematodes of cattleVeterinary Parasitology, Vol. 97, No. 3Detection of Mycobacterium bovis in Bovine Clinical Specimens Using Real-Time Fluorescence and Fluorescence Resonance Energy Transfer Probe Rapid-Cycle PCRJournal of Clinical Microbiology, Vol. 39, No. 4 Vol. 27, No. 1 Follow us on social media for the latest updates Metrics Downloaded 142 times History Published online 22 August 2018 Published in print July 1999 Information© 2018 Author(s)PDF download