Abstract
The intracellular localization of transfer RNA methylases has been investigated. In contrast to previous findings with nuclei from aqueous rat liver homogenates, non-aqueous tissue fractionation results in nuclei with a methylating activity 30 times higher than the corresponding cytoplasm. During fractionation, methylating activity rises parallel to the DNA content of the fraction. Methylases from non-aqueous nuclei and those known from the supernatants of aqueous homogenates have the same substrate specificity. It is concluded that the bulk of tRNA methylases is located within the nucleus of the cell.
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