Abstract
Methylation of tRNA in sea urchin Strongylocentrotus purpuratus, during embryogenesis was studied by labeling with [ Me- 3H]methionine. The changes in methylated bases in tRNA following fertilization were not uniform. The N 2-monomethylguanine was reduced to half and methylated uridylic acids increased 3-fold. Extracts of eggs and of embryos of various stages after fertilization were active in incorporating methyl groups from S- adenosyl- l -[Me- 14 C]methionine into Escherichia coli B tRNA.
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