Abstract
Objective To establish an effective method for inducing mouse bone marrow mesen-ehymal stem cells (MSCs) into hepatocytes. Methods Isolated MSCs were selected by plastic adher-ence, differentiated in Iscove's Modified Dulbecco's medium supplemented with 10% new bovine ser-um (NBS), 20 ng/mL hepatocyte growth factor (HGF), 10 ng/mL fibroblast growth factor-4 (FGF-4) and 10 ng/ml oncostatin m (OSM) for 20 days'induction. The medium was changed every 4 days.Results When MSCs were cultured with HGF, FGF-4 and OSM, cuboidal morphology, which was a characteristic of hepatocytes, was observed, and the differentiated ceils also expressed marker genes specific to liver cells in a time-dependent manner, a-fetoprotein (AFP) was expressed on day 10, and CK18, ALB and TAT were detected on day 20, which was in consistent with the immunofluoresence results. Differentiated cells further demonstrated these cells also acquired functional characteristics of hepatocytes of storing glycogen. Conclusion Mouse MSCs can differentiate into hepatocytes when in-duced by HGF, FGF-4 and OSM. Key words: Mesenchymal; stem; cell; Hepatocyte; Differentiation; HGF; FGF-4; OSM
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