Abstract
The WD40 transcription factor (TF) family is widespread in plants and plays important roles in plant growth and development, transcriptional regulation, and tolerance to abiotic stresses. WD40 TFs have been identified and characterized in a diverse series of plant species. However, little information is available on WD40 genes from D. longan. In this study, a total of 45 DlWD40 genes were identified from D. longan RNA-Seq data, and further analysed by bioinformatics tools. Also, the expression patterns of DlWD40 genes in roots and leaves, as well as responses to heat stress, were evaluated using quantitative real-time PCR (qRT-PCR). We found that the 45 DlWD40 proteins, together with 80 WD40 proteins from Arabidopsis and Zea mays, could be categorized into six groups. Of these, the DlWD40-4 protein was highly homologous to Arabidopsis WDR5a, a protein participating in tolerance to abiotic stresses. Moreover, a total of 25 cis-acting elements, such as abiotic stress and flavonoid biosynthesis elements, were found in the promoters of DlWD40 genes. The DlWD40-33 gene is targeted by miR3627, which has been proposed to be involved in flavonoid biosynthesis. Using qRT-PCR, ten of the 45 DlWD40 genes were demonstrated to have diverse expression patterns between roots and leaves, and these ten DlWD40 genes could also respond to varying durations of a 38 °C heat stress in roots and leaves. The results reported here will provide a basis for the further functional verification of DlWD40 genes in D. longan.
Highlights
Dimocarpus longan Lour. (D. longan), a member of the Sapindaceae family, is a perennial evergreen plant that is widely planted in humid and warm subtropical circumstances (Wang et al, 2015)
Genes from the D. longan genome that were highly homologous to the 45 DlWD40 genes identified from RNA-Seq data were used as representatives to study the DlWD40 gene structures and cis-acting elements
Identification and structural analysis of DlWD40 transcription factor (TF) from D. longan Based on Nr annotation, we obtained 45 putative DlWD40 genes from RNA-Seq data of D. longan leaves and roots
Summary
Dimocarpus longan Lour. (D. longan), a member of the Sapindaceae family, is a perennial evergreen plant that is widely planted in humid and warm subtropical circumstances (Wang et al, 2015). (D. longan), a member of the Sapindaceae family, is a perennial evergreen plant that is widely planted in humid and warm subtropical circumstances (Wang et al, 2015). D. longan is one of the major commercial crops in South China, Thailand, Vietnam, and other Southeast Asian countries, Received: 14 Dec 2020. Received in revised form: 06 Feb 2021. From Volume 49, Issue 1, 2021, Notulae Botanicae Horti Agrobotanici Cluj-Napoca journal will use article numbers in place of the traditional method of continuous pagination through the volume. The journal will continue to appear quarterly, as before, with four annual numbers
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