Abstract
BackgroundBacillus pumilus can secret abundant extracellular enzymes, and may be used as a potential host for the industrial production of enzymes. It is necessary to understand the metabolic processes during cellular growth. Here, an RNA-seq based transcriptome analysis was applied to examine B. pumilus BA06 across various growth stages to reveal metabolic changes under two conditions.ResultsBased on the gene expression levels, changes to metabolism pathways that were specific to various growth phases were enriched by KEGG analysis. Upon entry into the transition from the exponential growth phase, striking changes were revealed that included down-regulation of the tricarboxylic acid cycle, oxidative phosphorylation, flagellar assembly, and chemotaxis signaling. In contrast, the expression of stress-responding genes was induced when entering the transition phase, suggesting that the cell may suffer from stress during this growth stage. As expected, up-regulation of sporulation-related genes was continuous during the stationary growth phase, which was consistent with the observed sporulation. However, the expression pattern of the various extracellular proteases was different, suggesting that the regulatory mechanism may be distinct for various proteases. In addition, two protein secretion pathways were enriched with genes responsive to the observed protein secretion in B. pumilus. However, the expression of some genes that encode sporulation-related proteins and extracellular proteases was delayed by the addition of gelatin to the minimal medium.ConclusionsThe transcriptome data depict global alterations in the genome-wide transcriptome across the various growth phases, which will enable an understanding of the physiology and phenotype of B. pumilus through gene expression.
Highlights
Bacillus pumilus can secret abundant extracellular enzymes, and may be used as a potential host for the industrial production of enzymes
Sporulation and extracellular protease activity To gain insights into the temporal transcriptome changes of Bacillus pumilus BA06, two sets of cultures were established in 50 -ml of MM and gelatin-amended minimal medium (GM) (MM plus 2% gelatin) in 250 ml flasks
In conclusion, an RNA-seq-based transcriptome analysis was first applied to B. pumilus BA06 to monitor the transcriptional profile over the entire growth course in a defined MM or GM medium
Summary
Bacillus pumilus can secret abundant extracellular enzymes, and may be used as a potential host for the industrial production of enzymes. An RNA-seq based transcriptome analysis was applied to examine B. pumilus BA06 across various growth stages to reveal metabolic changes under two conditions. A combined omics-based approach has been applied to B. pumilus to try to understand cell physiology in response to oxidative stress and protein secretion [15, 16]. For this purpose, RNA-seq -based transcriptomics analysis is one of the most powerful tools that provides important insights into the functional elements of the genome, gene expression patterns and regulation [17], and offers a simpler and more cost-effective approach [18]. B. subtilis [19, 20], B. licheniformis [21], and B. thuringiensis [22] were examined by RNA-seq analysis
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
