Abstract
BackgroundValeriana fauriei is commonly used in the treatment of cardiovascular diseases in many countries. Several constituents with various pharmacological properties are present in the roots of Valeriana species. Although many researches on V. fauriei have been done since a long time, further studies in the discipline make a limit due to inadequate genomic information. Hence, Illumina HiSeq 2500 system was conducted to obtain the transcriptome data from shoot and root of V. fauriei.ResultsA total of 97,595 unigenes were noticed from 346,771,454 raw reads after preprocessing and assembly. Of these, 47,760 unigens were annotated with Uniprot BLAST hits and mapped to COG, GO and KEGG pathway. Also, 70,013 and 88,827 transcripts were expressed in root and shoot of V. fauriei, respectively. Among the secondary metabolite biosynthesis, terpenoid backbone and phenylpropanoid biosynthesis were large groups, where transcripts was involved. To characterize the molecular basis of terpenoid, carotenoid, and phenylpropanoid biosynthesis, the levels of transcription were determined by qRT-PCR. Also, secondary metabolites content were measured using GC/MS and HPLC analysis for that gene expression correlated with its accumulation respectively between shoot and root of V. fauriei.ConclusionsWe have identified the transcriptome using Illumina HiSeq system in shoot and root of V. fauriei. Also, we have demonstrated gene expressions associated with secondary metabolism such as terpenoid, carotenoid, and phenylpropanoid.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-016-2616-3) contains supplementary material, which is available to authorized users.
Highlights
Valeriana fauriei is commonly used in the treatment of cardiovascular diseases in many countries
Transcripts were highly involved in terpenoid backbone biosynthesis and phenylpropanoid biosynthesis compared to other biosynthesis by grouping into KEGG secondary metabolite biosynthetic pathway
We found that de novo transciptome sequencing with digital gene expression (DGE) analysis is a modern novel technique for the identification of specific genes for the candidate enzymes involved in the biosynthesis of secondary metabolites in V. fauriei
Summary
Valeriana fauriei is commonly used in the treatment of cardiovascular diseases in many countries. Several constituents with various pharmacological properties are present in the roots of Valeriana species. The roots have been known to contain several photochemical constituents with pharmacological properties including hypnotic, sedative, antispasmodic, mild anodyne, hypotensive and carminative effects [3]. One of the major secondary metabolites in medicinal plants, have many volatile representatives such as isoprenes (C5), monoterpenes (C10), sesquiterpenes (C15), even some diterpenes (C20), and triterepenes (C30) [10]. Members of this family serve many important functions like protecting the plants from many
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
