Abstract
BackgroundWoodland strawberry (Fragaria vesca) infected with Strawberry vein banding virus (SVBV) exhibits chlorotic symptoms along the leaf veins. However, little is known about the molecular mechanism of strawberry disease caused by SVBV.MethodsWe performed the next-generation sequencing (RNA-Seq) study to identify gene expression changes induced by SVBV in woodland strawberry using mock-inoculated plants as a control.ResultsUsing RNA-Seq, we have identified 36,850 unigenes, of which 517 were differentially expressed in the virus-infected plants (DEGs). The unigenes were annotated and classified with Gene Ontology (GO), Clusters of Orthologous Group (COG) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses. The KEGG pathway analysis of these genes suggested that strawberry disease caused by SVBV may affect multiple processes including pigment metabolism, photosynthesis and plant-pathogen interactions.ConclusionsOur research provides comprehensive transcriptome information regarding SVBV infection in strawberry.Electronic supplementary materialThe online version of this article (doi:10.1186/s12985-016-0584-5) contains supplementary material, which is available to authorized users.
Highlights
Woodland strawberry (Fragaria vesca) infected with Strawberry vein banding virus (SVBV) exhibits chlorotic symptoms along the leaf veins
Woodland strawberry infected with SVBV shows such characteristic symptoms as yellowing along the major leaf veins, shorter stolons, reduced plant growth and smaller fruit, as well as significant reduction in berry yield and quality
Illumina sequencing and reads assembly We chose strawberry plants infected with SVBV as a model to investigate host transcriptome responses
Summary
Using RNA-Seq, we have identified 36,850 unigenes, of which 517 were differentially expressed in the virus-infected plants (DEGs). The unigenes were annotated and classified with Gene Ontology (GO), Clusters of Orthologous Group (COG) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses. The KEGG pathway analysis of these genes suggested that strawberry disease caused by SVBV may affect multiple processes including pigment metabolism, photosynthesis and plant-pathogen interactions
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