Abstract
NAC domain genes belong to a large plant-specific transcription factor family, which is well-known to be associated with multiple stress responses and plant developmental processes. In this study, we screened differentially expressed genes (DEGs) and detected mRNA abundance of NAC family by RNA-Seq in the poplar leaves under salt stress condition. A total of 276 up-regulated DEGs and 159 down-regulated DEGs were identified to be shared in Populus alba × Populus glandulosa and Populus simonii × Populus nigra. Among 170 NAC members, NAC57 gene was significantly up-regulated in response to salt stress in the two species. Tissue-specific and salt-responsive analyses indicated the expression pattern of NAC57 gene was spatial and temporal in poplar under salt stress. Particle bombardment results showed subcellular localization of NAC57 was not solely nucleus-targeted. Full-length cDNA sequence of the NAC57 gene was cloned from P. alba × P. glandulosa and transformed into Arabidopsis thaliana. Under salt stress, transgenic Arabidopsis overexpressing NAC57 showed higher seed germination rate, root length, and fresh weight than wild type plants. In addition, the transgenic plants displayed higher superoxide dismutase activity and peroxidase activity, and lower malondialdehyde content and relative electrical conductivity than the wild type under salt stress condition. Furthermore, histochemical staining indicated reactive oxygen species accumulation was lower in the transgenic plants than that in the wild type under salt stress. All the results indicated that the NAC57 gene plays an important role in salt stress responses.
Highlights
Salinity is one of the most challenging abiotic stresses that threaten plant growth and development
The differentially expressed genes (DEGs) in P. alba × P. glandulosa under salt stress were profiled by RNA-Seq (Supplementary Data Sheet S2)
A total of 65535 Populus genes were detected in the RNA-Seq data, and as many as 2198 DEGs (FC ≥ 2, corrected p-value ≤ 0.05) including 901 up-regulated genes and 1297 down-regulated genes (Supplementary Data Sheet S2) were identified based on Pop’s Pipes software (Li et al, 2014)
Summary
Salinity is one of the most challenging abiotic stresses that threaten plant growth and development. Recent studies on plant abiotic stress responses were primarily focused on TFs regulation, ABA receptor identification, signal transduction and phytohormone mediation (Qin et al, 2011). TFs act as master regulators by activating or repressing a large number of functional genes through binding to stress-related cis-elements in the promoters of downstream. Understanding regulatory mechanism of TFs will facilitate deciphering complex signaling networks in plant stress responses (Shao et al, 2015). The networks of TFs with roles in environmental stress responses are unraveling and hundreds of genes encoding TFs participated in abiotic stress responses have been identified in recent years (Chen and Zhu, 2004)
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