Transcriptional response to cold and fasting acclimation in Onychostoma macrolepis during the overwintering stage

Abstract

In this study, we investigated the transcriptome responses of the liver of Onychostoma macrolepis in by RNA sequencing. The sampling process involved three groups: 1G (0 week, 10 °C), 2G (12 weeks, 0 °C) and 3G (24 weeks, 10 °C). The body weight, viscera index, hepatopancreas index and intraperitoneal fat index of O. macrolepis showed a decreasing trend with the prolonging of overwintering time. The crude fat contents of whole fish, muscle and liver in O. macrolepis after overwintering were significantly lower than those of the fish before overwintering (p < 0.05). In 1G versus 2G group, 2G versus 3G group and 1G versus 3G group, the differently expressed genes (DEGs) were 4630, 3976 and 2311, respectively. These results indicated that different stages of overwintering period had significant effects on gene expression of O. macrolepis, and the influence degree gradually decreased with the extension of overwintering period. The results of Gene ontology (GO) enrichment showed that these DEGs were mainly related to metabolism and immunity, and most of them were down-regulated. In this study, the KEGG pathway classification results showed that signal transduction was the most representative. In addition, KOG enrichment results showed that many DEGs associated with lipid transport and metabolism were down-regulated during the overwintering period. These observations suggested that slowing metabolism and delaying immunity may be the strategies for overwintering adaptation of O. macrolepis.