Abstract
The promoter of rat multidrug resistance protein 3 (Mrp3) has been cloned and analyzed in the rat intestinal cell line (IEC-18 cells). A series of 5′ deletion mutants of the Mrp3 promoter region were constructed and placed into the pGL3-Basic vector (luciferase reporter gene). Deletion analysis of the Mrp3 promoter identified a basal transcription element at −123/−106, two negative response regions at −2723/−1128 and −530/−443, respectively, and two positive response regions at −1063/−943 and −302/−157. Further site-directed mutagenesis analysis and gel mobility shift assays provided evidence for Sp1 and Sp3 binding within −123/−105 regions. These studies indicate that Sp1 and Sp3 may be involved in the regulation of the rat Mrp3 gene.
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