Abstract
MoHrip1 is a protein elicitor isolated from Magnaporthe oryzae and was found to induce blast-resistance in rice. To investigate the comprehensive functions of MoHrip1, next-generation sequencing (NGS)-based digital gene expression (DGE) profiling was performed to collect the transcriptional data of differentially expressed genes (DEGs) induced by MoHrip1. A total of 308 genes were identified with differential expression, and 80 genes were predicted to be induced specifically by MoHrip1. Among these 308 genes, a series of genes associated with the salicylic acid (SA) pathway, phytoalexin, transcription factors, and pathogen-related proteins were identified. Both the SA signaling pathway and the gibberellin (GA) pathway were activated, while the jasmonic acid (JA) signaling pathway was repressed. The contents of endogenous SA and GA and the morphological characteristics of the rice after treatment were measured to provide evidence supporting the predictions made based on the DGE data. The 80 genes mentioned above might be candidate genes for studying interactions with MoHrip1. The transcriptional data provided global effect information in rice induced by MoHrip1, and all the results demonstrated that MoHrip1 could induce pathogen resistance and promote plant growth by regulating the contents of SA and GA directly or indirectly.
Highlights
Rice blast, a major fungal disease caused by Magnaporthe oryzae, seriously affects rice production worldwide (Ribot et al, 2008), leading to yield losses of 50–70% (Bagnaresi et al, 2012)
To obtain the global gene expression profile differences of rice treated with MoHrip1 compared to those treated with buffer (Tris-Cl) as a negative control, 24, 48, and 72 hat and non-treated rice samples were prepared and sequenced on the Illumina sequencing platform
We present an RNA-seq study focusing on the response of rice to secretion protein elicitors
Summary
A major fungal disease caused by Magnaporthe oryzae, seriously affects rice production worldwide (Ribot et al, 2008), leading to yield losses of 50–70% (Bagnaresi et al, 2012). As rice is the world’s second most important human food crop (FAOSTAT, 2014), it is necessary to develop methods to protect it from pathogenic diseases and to increase its production. Increasing research on the molecular events governing the interactions between rice and M. oryzae have contributed to methods for improving disease resistance (Chen and Ronald, 2011). Some elicitors have been used to improve the pathogen resistance of plants (Bent and Mackey, 2007). Such elicitor molecules include oligosaccharides, lipids, peptides, glycoproteins, and proteins (Nürnberger, 1999; De Wit et al, 2009; Ellis et al, 2009)
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
