Transcriptional profiling of receptor-transporting protein 3 variants in European sea bass and gilthead seabream suggests divergent roles.

Viral nervous necrosis outbreaks caused by the RGNNV/SJNNV reassortant betanodavirus in gilthead sea bream (Sparus aurata) and European sea bass (Dicentrarchus labrax)

Successful cryopreservation in biodegradable containers of sperm from aquaculture Mediterranean fishes

Circadian feeding schedules in gilthead sea bream (Sparus aurata) and European sea bass (Dicentrarchus labrax): A comparative approach towards improving dietary fish oil utilization and n-3 LC-PUFA metabolism

From operculum and body tail movements to different coupling of physical activity and respiratory frequency in farmed gilthead sea bream and European sea bass. Insights on aquaculture biosensing

In this study, 50 000 European seabass and 50 000 gilthead seabream with an average wet weight of 1.6 g were cultivated during 600 days under low water salinity conditions (7‰) of which getting out from underground at fixed 19°C, being produced in earthen ponds under commercial production conditions. From the beginning to the end of the experiment, the same fish feeds were used for both species throughout the study, and when European seabass reached 328.4±22.98 g, gilthead seabream reached to 369.12±24.11 g. At the end of the experiment, while the feed conversion rate (FCR) was calculated as 1.72±0.06 for European sea bass, it was calculated 1.53±0.03 for gilthead sea bream. Protein efficiency ratios were 1.24±0.17 for European seabass, and 1.40±0.06 for gilthead seabream. Significant differences were found between two species in terms of live weight and FCR (p 0.05). The results showed that gilthead seabream taken into earthen ponds and low salinity brackish water was able to reach 300 g and over live weight with faster and lower FCR values compared to European sea bass in the certain time.

Comparative efficacy of clove oil and 2-phenoxyethanol as anesthetics in the aquaculture of European sea bass ( Dicentrarchus labrax) and gilthead sea bream ( Sparus aurata) at different temperatures

Virulence and Molecular Typing of Vibrio harveyi Strains Isolated from Cultured Dentex, Gilthead Sea Bream and European Sea Bass

Rearing temperature enhances hepatic glucokinase but not glucose-6-phosphatase activities in European sea bass ( Dicentrarchus labrax) and gilthead sea bream ( Sparus aurata) juveniles fed with the same level of glucose

Antibacterial and anti-PAF activity of lipid extracts from sea bass (Dicentrarchus labrax) and gilthead sea bream (Sparus aurata)

The aim of the present study was the investigation of the biological cycle of the isopod parasite Ceratothoa oestroides (Risso, 1836), the way of installation in the host and the study of its pathology in young individuals of gilthead sea bream {Sparus aurata) and sea bass {Dicentrarchus labrax) raised in experimental aquariums. During the experiment, initially, 10 sea bass of marketable size (300-400gr) were collected infected with lice from the region of Chios and Epidavros. The lice were mature and gravid. The adults and their hosts were kept in 151t aquariums, with constant water of 32-33%c salinity at temperature of 21-22° C, until they would give both to young parasites. The time that parasites stayed in aquarium was three months. After three months, new hatched larvae appeared. The pulii II stage, which was responsible for the most alterations that were observed in the fish and particular in sea bass, were observed in the first week, after the hatching. In this stage, parasites swam freely in the surface of water column. At this phase, 100 parasites were transported in 2 different aquariums with the same conditions of salinity and temperature as in the first aquarium, where young uninfected individuals (3-5 gr) of gilthead sea bream {Sparus aurata) and sea bass {Dicentrarchus labrax) were placed. The number of the uninfected, healthy fish was 40 individuals per aquarium. During the duration of experiment four weekly samplings of five individuals per sample were performed and these underwent a macroscopical, parasitological and histopathological examination. In addition, the mortalities that existed and the final luck of parasites afterwards the death of their hosts were recorded. From the results, we concluded that pulii II stage caused serious lesions and eventually the death of mainly young fish, gilthead sea bream {Sparus aurata) and sea bass {Dicentrarchus labrax). The process from the moment of "invasion" of young parasites until their final installation in the buccal cavity lasted around 2 hours. In one week, all the isopods were installed in the buccal cavity of young gilthead sea bream {Sparus aurata) and sea bass {Dicentrarchus labrax). During the second week, the first symptoms of fish appeared. The infected fish swam fast and rubbed their body against the aquarium surface in order to remove the parasites. In the sea bass {Dicentrarchus labrax), with the appearance of first symptoms, the biggest rate of mortality (56,25%) followed, while in the gilthead sea bream {Sparus aurata), the biggest rate of mortality (52%) was observed in the third week. With regard to the luck of the young parasites that remained without host it was observed that they swam for 48 hours, then fell in the bottom of aquarium and after 24 hours the first mortalities started. The adult parasites, that gave the young pulii II after 24 hours, were dead in the bottom of aquarium.

Production and characterization of recombinantly derived peptides and antibodies for accurate determinations of somatolactin, growth hormone and insulin-like growth factor-I in European sea bass ( Dicentrarchus labrax)

Effect of mechanical separation process on lipid oxidation in European aquacultured sea bass, gilthead sea bream, and rainbow trout products

The study of melatonin is of great importance for the fundamental knowledge of any living system since it displays many different physiological roles, including being a potent natural antioxidant. To the best of our knowledge, there is no information regarding melatonin in fish seminal plasma. This study aimed to determine this clock-hormone levels in the seminal plasma of three aquaculture fish species: European seabass (Dicentrarchus labrax), gilthead seabream (Sparus aurata), and Senegalese sole (Solea senegalensis) (both wild and F1 breeders), and to explore melatonin potential role in fish reproduction and spermatozoa antioxidant status. Blood and seminal plasma were collected from fish during their reproductive season, at two different times of the day [mid-light (ML) and mid-dark (MD)], and melatonin concentration was determined by radioimmunoassay (RIA). Testosterone (T), 11-ketotestosterone (11KT), and total antioxidant status (TAS) were also determined, to investigate the putative role of seminal melatonin in fish reproduction, both at endocrine and antioxidant levels. For each species, Pearson's correlation analysis was performed between all possible factors. Blood plasma melatonin showed higher average values at night in the three species: gilthead seabream (808 ± 139 pg/mL), European seabass (364 ± 85 pg/mL), and Senegalese sole (248 ± 40 and 88 ± 11 pg/mL in F1 and wild males, respectively). However, melatonin levels in seminal plasma were species-specific: in European seabass, melatonin levels were not detectable at any time-point, whereas in gilthead seabream it was only found at MD (average of 21 pg/mL), and in Senegalese sole, different melatonin patterns were observed between F1 and wild males, but both had higher melatonin at MD (6.84 and 14.26 pg/mL, respectively). In gilthead seabream, at MD seminal melatonin levels correlated with the antioxidant status of seminal plasma. A relationship between blood melatonin and seminal TAS levels was observed in European seabass at ML: in this species, seminal melatonin could not be detected and the lowest seminal TAS levels were found. Regarding steroid analysis, opposite patterns in the seminal plasma of F1 and wild Senegalese sole were observed: at MD, wild Senegalese sole had substantially greater 11KT levels (2.53 ng/mL), whereas F1 males had higher T levels (1.92 ng/mL). In gilthead seabream, a positive correlation between T and ML blood melatonin and seminal TAS was observed. This study unraveled the species-specificity and daily changes of melatonin in fish seminal plasma.

Nodavirus infection induces a great innate cell-mediated cytotoxic activity in resistant, gilthead seabream, and susceptible, European sea bass, teleost fish

Disease outbreaks are a major threat to the aquaculture industry, and can be controlled by selective breeding. With the development of high-throughput genotyping technologies, genomic selection may become accessible even in minor species. Training population size and marker density are among the main drivers of the prediction accuracy, which both have a high impact on the cost of genomic selection. In this study, we assessed the impact of training population size as well as marker density on the prediction accuracy of disease resistance traits in European sea bass (Dicentrarchus labrax) and gilthead sea bream (Sparus aurata). We performed a challenge to nervous necrosis virus (NNV) in two sea bass cohorts, a challenge to Vibrio harveyi in one sea bass cohort and a challenge to Photobacterium damselae subsp. piscicida in one sea bream cohort. Challenged individuals were genotyped on 57K–60K SNP chips. Markers were sampled to design virtual SNP chips of 1K, 3K, 6K, and 10K markers. Similarly, challenged individuals were randomly sampled to vary training population size from 50 to 800 individuals. The accuracy of genomic-based (GBLUP model) and pedigree-based estimated breeding values (EBV) (PBLUP model) was computed for each training population size using Monte-Carlo cross-validation. Genomic-based breeding values were also computed using the virtual chips to study the effect of marker density. For resistance to Viral Nervous Necrosis (VNN), as one major QTL was detected, the opportunity of marker-assisted selection was investigated by adding a QTL effect in both genomic and pedigree prediction models. As training population size increased, accuracy increased to reach values in range of 0.51–0.65 for full density chips. The accuracy could still increase with more individuals in the training population as the accuracy plateau was not reached. When using only the 6K density chip, accuracy reached at least 90% of that obtained with the full density chip. Adding the QTL effect increased the accuracy of the PBLUP model to values higher than the GBLUP model without the QTL effect. This work sets a framework for the practical implementation of genomic selection to improve the resistance to major diseases in European sea bass and gilthead sea bream.