Abstract
SummaryPdgfra+ oligodendrocyte precursor cells (OPCs) arise in distinct specification waves during embryogenesis in the central nervous system (CNS). It is unclear whether there is a correlation between these waves and different oligodendrocyte (OL) states at adult stages. Here, we present bulk and single-cell transcriptomics resources providing insights on how transitions between these states occur. We found that post-natal OPCs from brain and spinal cord present similar transcriptional signatures. Moreover, post-natal OPC progeny of E13.5 Pdgfra+ cells present electrophysiological and transcriptional profiles similar to OPCs derived from subsequent specification waves, indicating that Pdgfra+ pre-OPCs rewire their transcriptional network during development. Single-cell RNA-seq and lineage tracing indicates that a subset of E13.5 Pdgfra+ cells originates cells of the pericyte lineage. Thus, our results indicate that embryonic Pdgfra+ cells in the CNS give rise to distinct post-natal cell lineages, including OPCs with convergent transcriptional profiles in different CNS regions.
Highlights
Oligodendrocytes (OLs; for abbreviations, please refer to Table S4) are one of the most abundant cell types in the central nervous system (CNS)
Post-natal oligodendrocyte precursor cells (OPCs) progeny of E13.5 platelet-derived growth factor receptor alpha (Pdgfra)+ cells present electrophysiological and transcriptional profiles similar to OPCs derived from subsequent specification waves, indicating that Pdgfra+ preOPCs rewire their transcriptional network during development
Our results indicate that embryonic Pdgfra+ cells in the CNS give rise to distinct post-natal cell lineages, including OPCs with convergent transcriptional profiles in different CNS regions
Summary
Oligodendrocytes (OLs; for abbreviations, please refer to Table S4) are one of the most abundant cell types in the central nervous system (CNS). OPCs expressing platelet-derived growth factor receptor alpha (Pdgfra) appeared to arise exclusively from ventral domains of the CNS, with a subset migrating to dorsal regions (Pringle and Richardson, 1993). This led to the hypothesis of a single embryonic lineage for OPCs, arising at embryonic day (E) 12.5 from progenitor domains and dependent on sonic hedgehog (Shh) (Richardson et al, 2000). The last wave of cortically derived Emx1-expressing precursors begins
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
