Abstract
The 5'-end of the bovine adrenodoxin gene contains unique structural characteristics. Exons 1 and 2 appear to encode different presequences for this mitochondrial precursor protein. However, exon 1 contains a stop codon (TAA) in place of amino acid 15. Thus, while two species of bovine adrenodoxin mRNA arise from a single gene, only the abundant mRNA species (90%) is translated into the adrenodoxin precursor which contains the presequence encoded by exon 2. The minor mRNA species (10%) contains the sequence encoded by exon 1 and cannot be translated into an adrenodoxin precursor. Presumably the sequence within exon 2 is removed from this minor mRNA species by alternative splicing. Furthermore, the initiation of transcription of the major adrenodoxin mRNA (exon 2) lies within intron 1 of this unusual gene, while that for the other mRNA (exon 1) lies in the 5'-flanking region. Thus, the adrenodoxin gene is the first example of a gene encoding a mitochondrial protein which falls into the category of genes having alternate promoters utilizing a pattern of alternative splicing.
Highlights
Transcription of the Bovine Adrenodoxin Gene ProducesTwo Species of mRNA of Which Only One Is Translated into Adrenodoxin”
(90%)is translatedintotheadrenodoxinprecursor mately 90% of the totaladrenodoxin mRNA in bovine adrenal which contains the presequence encoded by exon 2. cortex
The initiation of transcriptionof the major adrenodoxin mRNA lies within intron 1 otfhis unusualgene, while that for the othmerRNA lieisn the5”flanking doxin mRNA are elevated in primary cultures of bovine adrenocortical cells upon treatment with ACTH,presumably as a resultof increased transcription mediated by CAMP [11].To establish the origin of these two distinct species of adrenodoxin mRNA we have characterized by sequence analysis the 5’-end of the bovine adrenodoxin gene and found it to contain a very unique structure
Summary
Transcription of the Bovine Adrenodoxin Gene ProducesTwo Species of mRNA of Which Only One Is Translated into Adrenodoxin”. Exon 1 containfosrms of adrenodoxin mRNA, both encoding a common maa stop codon (TAA) in place of amino acid 15.
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