Transcription initiation sites for the potato mitochondrial gene coding for subunit 9 of ATP synthase ( atp9)

Abstract

The potato mitochondrial atp9 gene has a simple expression pattern. To determine where transcription initiates, primary mitochondrial RNAs were labeled by in vitro capping and hybridized to the 5′ flanking sequences of the atp9 gene. A single transcription initiation region was identified. Primer extension and nuclease S1 protection analyses were used to precisely map the transcript 5′ termini in this region. These results indicate that transcription initiates at 121–128 bp upstream of the atp9 open reading frame, in a sequence which does not present any homology with proposed consensus sequences for plant mitochondrial promoters. Nuclease S1 protection were also used to map 3′ termini 67–71 nucleotides downstream of a putative single-stem loop structure.