Tracing early biomineralization: bidirectional foliated shell microstructures in early Cambrian molluscs

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Understanding the microstructural diversity of mineralized shells in early molluscs is essential for reconstructing the origin of the phylum during the Cambrian Explosion. Here, we report the first occurrence of a bidirectional foliated aragonite microstructure in early molluscs from the Cambrian Terreneuvian Bayangol Formation in the Zavkhan Basin of southwestern Mongolia. This microstructural type, previously known only in Cambrian hyolith skeletons, is documented for the first time through detailed microstructural characterization of shells in three helcionelloid species: Bemella simplex, Latouchella korobkovi and Merismoconcha tommotica. Our study significantly expands the recognized diversity of shellmicrostructures in early molluscs, reinforcing the skeletal commonality and homology of biomineralization mechanisms shared byhelcionelloids and hyoliths. Xia Baige [xiabaige@stumail.nwu.edu.cn], Shaanxi Key Laboratory of Early Life and Environments, State Key Laboratory of Continental Dynamics and Department of Geology, Northwest University, Xi’an 710069, PR China; Li Luoyang* [liluoyang@ouc.edu.cn], Key Lab of Submarine Geosciences and Prospecting Techniques, Ministry of Education and College of Marine Geosciences, Ocean University of China, Qingdao 266100, PR China.

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  • Cite Count Icon 23
  • 10.1111/are.13265
Effects of low dietary fish meal on the volatile compounds in muscle of large yellow croaker Larimichthys crocea
  • Apr 7, 2017
  • Aquaculture Research
  • Hua Mu + 5 more

Aquaculture ResearchVolume 48, Issue 9 p. 5179-5191 Short Communication Effects of low dietary fish meal on the volatile compounds in muscle of large yellow croaker Larimichthys crocea Hua Mu, Hua Mu The Key Laboratory of Aquaculture Nutrition and Feeds, Ministry of Agriculture; The Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao, ChinaThese authors contributed equally to this work.Search for more papers by this authorZehong Wei, Zehong Wei The Key Laboratory of Aquaculture Nutrition and Feeds, Ministry of Agriculture; The Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao, ChinaThese authors contributed equally to this work.Search for more papers by this authorLina Yi, Lina Yi The Key Laboratory of Aquaculture Nutrition and Feeds, Ministry of Agriculture; The Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao, ChinaSearch for more papers by this authorJikang Shentu, Jikang Shentu Ningbo Ocean and Fisheries Research Institute, Ningbo, ChinaSearch for more papers by this authorWenbing Zhang, Corresponding Author Wenbing Zhang wzhang@ouc.edu.cn The Key Laboratory of Aquaculture Nutrition and Feeds, Ministry of Agriculture; The Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao, ChinaCorrespondence: W Zhang, The Key Laboratory of Aquaculture Nutrition and Feeds, Ministry of Agriculture; The Key Laboratory of Mariculture, Ministry of Education; Ocean University of China, Qingdao 266003, China. E-mail: wzhang@ouc.edu.cnSearch for more papers by this authorKangsen Mai, Kangsen Mai The Key Laboratory of Aquaculture Nutrition and Feeds, Ministry of Agriculture; The Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao, ChinaSearch for more papers by this author Hua Mu, Hua Mu The Key Laboratory of Aquaculture Nutrition and Feeds, Ministry of Agriculture; The Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao, ChinaThese authors contributed equally to this work.Search for more papers by this authorZehong Wei, Zehong Wei The Key Laboratory of Aquaculture Nutrition and Feeds, Ministry of Agriculture; The Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao, ChinaThese authors contributed equally to this work.Search for more papers by this authorLina Yi, Lina Yi The Key Laboratory of Aquaculture Nutrition and Feeds, Ministry of Agriculture; The Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao, ChinaSearch for more papers by this authorJikang Shentu, Jikang Shentu Ningbo Ocean and Fisheries Research Institute, Ningbo, ChinaSearch for more papers by this authorWenbing Zhang, Corresponding Author Wenbing Zhang wzhang@ouc.edu.cn The Key Laboratory of Aquaculture Nutrition and Feeds, Ministry of Agriculture; The Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao, ChinaCorrespondence: W Zhang, The Key Laboratory of Aquaculture Nutrition and Feeds, Ministry of Agriculture; The Key Laboratory of Mariculture, Ministry of Education; Ocean University of China, Qingdao 266003, China. E-mail: wzhang@ouc.edu.cnSearch for more papers by this authorKangsen Mai, Kangsen Mai The Key Laboratory of Aquaculture Nutrition and Feeds, Ministry of Agriculture; The Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao, ChinaSearch for more papers by this author First published: 07 April 2017 https://doi.org/10.1111/are.13265Citations: 9 Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat Citing Literature Volume48, Issue9September 2017Pages 5179-5191 RelatedInformation

  • Research Article
  • Cite Count Icon 4
  • 10.1111/1755-6724.14579
Discovery of Neoproterozoic Rocks in the Dunhuang Block: Clues to Correlation with Rodinia
  • Aug 1, 2020
  • Acta Geologica Sinica - English Edition
  • Tingyi Wang + 3 more

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  • Research Article
  • Cite Count Icon 2
  • 10.1111/1755-6724.14662
Discovery of Multiple Tectonic Reformations of the Eastern Yingen‐Ejinaqi Basin: Evidence from Detrital Chronology
  • Apr 1, 2021
  • Acta Geologica Sinica - English Edition
  • Heng Peng + 8 more

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  • Front Matter
  • 10.1016/j.gr.2009.12.004
International Association for Gondwana Research 2010 Annual Convention and Gondwana to Asia symposium, Qingdao, China
  • Dec 21, 2009
  • Gondwana Research
  • Sanzhong Li

International Association for Gondwana Research 2010 Annual Convention and Gondwana to Asia symposium, Qingdao, China

  • Research Article
  • 10.3724/sp.j.1118.2018.17258
Expression character analysis of the stress granule marker TIA-1 in the intestinal cells of sea cucumber in response to heat stress
  • Jan 1, 2018
  • Journal of Fishery Sciences of China
  • Shasha Wang + 3 more

PDF HTML阅读 XML下载 导出引用 引用提醒 高温胁迫下刺参肠道细胞应激颗粒标记蛋白TIA-1的表达特征 DOI: 作者: 作者单位: 1. 中国海洋大学 水产学院, 海水养殖教育部重点实验室, 山东 青岛 266003;2. 浙江海洋大学 海洋科学与技术学院, 海洋生物种质资源发掘利用国家地方联合工程实验室, 浙江 舟山 316000;3. 中国海洋大学 水产学院, 水产病害与免疫学研究室, 山东 青岛 266003 作者简介: 王莎莎(1991-),女,硕士研究生,从事海洋无脊椎动物分子生物学研究.E-mail:wangshashaabcd@163.com 通讯作者: 中图分类号: S917 基金项目: 国家自然科学基金项目(31472257). Expression character analysis of the stress granule marker TIA-1 in the intestinal cells of sea cucumber in response to heat stress Author: Affiliation: 1. The Key Laboratory of Mariculture(Ocean University of China), Ministry of Education, Qingdao 266003, China;2. National and Local Engineering Laboratory of the Exploitation and Utilization of Marine Biological Germplasm Resources, Marine Science and Technology College, Zhejiang Ocean University, Zhoushan 316000, China;3. Laboratory of Pathology and Immunology of Aquatic Animals, College of Fisheries, Ocean University of China, Qingdao 266003, China Fund Project: 摘要 | 图/表 | 访问统计 | 参考文献 | 相似文献 | 引证文献 | 资源附件 | 文章评论 摘要:产生应激颗粒是生物体遭受不利环境时细胞产生的一种自我保护机制,基于此,本研究开展了高温胁迫下刺参(表达特征的研究。采用RACE技术克隆了刺参T细胞胞内抗原-1基因()全长cDNA序列。该基因cDNA全长为3108 bp,包括16 bp的5'UTR,1284 bp的开放阅读框(ORF),1808 bp的3'UTR,编码427个氨基酸。结构分析表明,刺参基因编码3个N末端RNA识别基序(RRM)和2个类似蛋白聚集区的C末端区域(low complexity)。系统进化树分析表明,刺参TIA-1与软体动物门鸭嘴海豆芽()TIAR聚为一支,具有最近的亲缘关系。使用Western blotting技术检测目的蛋白TIA-1在全细胞和亚细胞成分中的表达差异,并且利用细胞免疫荧光技术检测细胞在高温胁迫下TIA-1的定位情况。研究结果表明,刺参肠道细胞TIA-1在高温胁迫下在细胞质中表达量显著上升(<0.05),且高温胁迫下TIA-1定位表达发生变化,多分布在细胞核周围,由此推断TIA-1在刺参高温应激时可能参与了应激颗粒的生成,从而对刺参肠道细胞起到了保护作用。 Abstract:In response to environmental stresses, such as heat shock, oxidative stress, and viral infection, arrest of translation initiation reduces energy-expensive cellular processes and conserves the energy required for the repair of cellular damage. Untranslated mRNAs that accumulate in these cells move to discrete cytoplasmic foci known as stress granules (SGs). SGs are characterized by the presence of translational initiation factors such as eIF4E, eIF4G, eIF4A, eIF4B, eIF3, eIF2, poly(A)-binding protein, and stalled 40S ribosomal units. The best-known proteins integral to SG formation are T-cell intracellular antigen (TIA) proteins, which are considered robust markers of SGs. The assembly of SGs, one of the most important post-transcriptional mechanisms, helps cells to survive under adverse environmental conditions. Currently, there is no information on the cell-protective roles of SGs in marine invertebrates under environmental stress. In the present study, we hypothesized that TIA-1, an mRNA-binding protein that can aggregate within granules, may play a significant role in the sea cucumber in response to heat stress. We characterized the amino sequence of TIA-1. The full-length cDNA of TIA-1 is 3108 bp, comprising a 5'-untranslated region (UTR) of 16 bp, 3'-UTR of 1808 bp, and open reading frame (ORF) of 1284 bp. The ORF encodes 427 amino acids, with a calculated molecular mass of 48.07 kD and 6.19 theoretical isoelectric point. Structural analysis showed that TIA-1 has three N-terminal RNA-recognition motifs and two C-terminal low-complexity regions, which are related to prion proteins and have the capacity to form reversible aggregates. Phylogenetic analyses revealed that TIAR. Moreover, we detected whole-cell and subcellular protein expression levels of TIA-1 by using western blotting. The localization of TIA-1 to subnuclear structures was assessed using immunofluorescence analysis. The results showed that the expression level of TIA-1 in cytoplasmic fractions under heat stress (25℃) was significantly increased when compared with the control group (15℃). The fluorescence signals of TIA-1 were observed to be translocated from the nucleus to the cytoplasm in response to heat stress, which suggests that TIA-1 may be involved in the formation of SGs to protect the stressed intestinal cells of under high temperature conditions. We identified a potential novel cytoprotective mechanism in response to heat stress in from the viewpoint of post-transcriptional regulation, which may play an important role in reducing the translational rates and/or mRNA processing under unfavorable environmental conditions in marine invertebrates. 参考文献 相似文献 引证文献

  • Book Chapter
  • Cite Count Icon 6
  • 10.5772/7102
Numerical Simulation of Flow in Erlenmeyer Shaken Flask
  • Jan 1, 2010
  • Liu Tianzhong + 4 more

Liu Tianzhong1, Su Ge2, Li Jing3, Qi Xiangming4 and Zhan Xiaobei3 1Qingdao Institute of Bioenergy and Bioprocess Technology, China Academy of Sciences,Qingdao 266101, 2College of Material Science and Engineering, Ocean University of China,Qingdao 266101, 3China Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, 4College of Food Science and Engineering, Ocean University of China,Qingdao 266003, China

  • Addendum
  • 10.1016/j.precamres.2014.08.012
WITHDRAWN: Ca. 2.5 billion year old mafic dykes in western Shandong Province: Implications for foundering of the lower crust within the North China Craton, China
  • Aug 1, 2014
  • Precambrian Research
  • Shen Liu + 6 more

WITHDRAWN: Ca. 2.5 billion year old mafic dykes in western Shandong Province: Implications for foundering of the lower crust within the North China Craton, China

  • Research Article
  • Cite Count Icon 22
  • 10.4028/www.scientific.net/kem.672.113
To Infer the early Evolution of Mollusc Shell Microstructures
  • Jan 1, 2016
  • Key Engineering Materials
  • Michael J Vendrasco + 4 more

To infer the early evolution of mollusc shell microstructures we must know the most ancient fossil record of molluscs. Fortunately the shells of many early molluscs are preserved via internal coatings and replacements by apatite that record sub-micrometer structural details that otherwise would be lost during diagenetic recrystallization. We herein discuss the methodology by which one can infer original shell microstructure from phosphatized fossils, pointing out the main problems and solutions in interpreting these traces of original shell crystal morphology. We also review the information these fossils have provided about the earliest evolution of the mollusc shell. Our long-term goal is to create a dataset of microstructures in early molluscs, which will be useful in understanding the incipient evolutionary arms race between molluscs and their predators, and will help elucidate how the mollusc biomineralization toolkit was built through time.

  • Research Article
  • Cite Count Icon 42
  • 10.1130/b31822.1
Linking high-pressure mafic granulite, TTG-like (tonalitic-trondhjemitic) leucosome and pluton, and crustal growth during continental collision
  • Nov 9, 2018
  • GSA Bulletin
  • Shengyao Yu + 5 more

Research Article| November 09, 2018 Linking high-pressure mafic granulite, TTG-like (tonalitic-trondhjemitic) leucosome and pluton, and crustal growth during continental collision Shengyao Yu; Shengyao Yu † 1Key Laboratory of Submarine Geosciences and Prospecting Technique, MOE and College of Marine Geosciences, Ocean University of China, Qingdao 266100, China2Laboratory for Marine Geology, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266061, China †yushengyao@ouc.edu.cn Search for other works by this author on: GSW Google Scholar Sanzhong Li; Sanzhong Li 1Key Laboratory of Submarine Geosciences and Prospecting Technique, MOE and College of Marine Geosciences, Ocean University of China, Qingdao 266100, China2Laboratory for Marine Geology, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266061, China Search for other works by this author on: GSW Google Scholar Jianxin Zhang; Jianxin Zhang 3Institute of Geology, Chinese Academy of Geological Sciences, Beijing 100037, China Search for other works by this author on: GSW Google Scholar Deyou Sun; Deyou Sun 4College of Earth Sciences, Jilin University, Changchun 130061, China Search for other works by this author on: GSW Google Scholar Yinbiao Peng; Yinbiao Peng 1Key Laboratory of Submarine Geosciences and Prospecting Technique, MOE and College of Marine Geosciences, Ocean University of China, Qingdao 266100, China Search for other works by this author on: GSW Google Scholar Yunshuai Li Yunshuai Li 5Institute of Surface-Earth System Science, Tianjin University, Tianjin 300072, China Search for other works by this author on: GSW Google Scholar GSA Bulletin (2019) 131 (3-4): 572–586. https://doi.org/10.1130/B31822.1 Article history received: 06 Apr 2017 rev-recd: 10 Jan 2018 accepted: 14 Aug 2018 first online: 10 Nov 2018 Cite View This Citation Add to Citation Manager Share Icon Share Facebook Twitter LinkedIn MailTo Tools Icon Tools Get Permissions Search Site Citation Shengyao Yu, Sanzhong Li, Jianxin Zhang, Deyou Sun, Yinbiao Peng, Yunshuai Li; Linking high-pressure mafic granulite, TTG-like (tonalitic-trondhjemitic) leucosome and pluton, and crustal growth during continental collision. GSA Bulletin 2018;; 131 (3-4): 572–586. doi: https://doi.org/10.1130/B31822.1 Download citation file: Ris (Zotero) Refmanager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentBy SocietyGSA Bulletin Search Advanced Search Abstract The early continental crust is composed predominantly of Archean tonalite-trondhjemite-granodiorite (TTG); however, the tectonic regime and melting conditions for TTG magmas have been debated. In this study, we report field and microscopic evidence for the partial melting of arc-related metagabbro and its products, including TTG-like melt, volumetrically significant plutons evolved from melt, and the associated granulitic residua during continental collision in the North Qaidam Mountains, China. Migmatite shows successive stages of initial intragranular or droplet-like melt along grain boundaries, which grew into a three-dimensional interconnected intergranular network, segregated, and accumulated in pressure shadow areas, and merged to form melt channels and sheets that finally combined to form a TTG-like tonalite pluton. Pressure-temperature (P-T) calculations indicate high-pressure granulite-facies metamorphism and the crystallization of leucosome at P = 15.5–18.5 kbar and T = 850–950 °C. Based on zircon U-Pb dating and petrological analyses, partial melting and magmatic crystallization occurred 438–430 m.y. ago, which is slightly younger or temporally overlaps with ultrahigh-pressure metamorphism in the region. The metagabbros exhibit a subduction-related arc signature with slightly positive εNd(t) values of 2.1–4.2. The felsic leucosomes and tonalite plutons are characterized by high Na, Sr, Sr/Y, and La/Yb values and low heavy rare earth element values, with εNd(t) values of 0.1–4.3, similar to typical TTGs. The geological context, geochemistry, and timing of the TTG-like melt formation observed in this study differ from the prevailing models; however, our observations and documentations demonstrate that melting of arc-like metagabbro under high-pressure granulite-facies conditions during continental collision may make important contributions to crustal growth and differentiation. You do not have access to this content, please speak to your institutional administrator if you feel you should have access.

  • Abstract
  • 10.1016/j.eujim.2014.09.100
Salvianolic acid A, a novel matrix metalloproteinase-9 inhibitor, reduces microvascular damage induced by hypertension
  • Nov 12, 2014
  • European Journal of Integrative Medicine
  • Fukang Teng + 7 more

Salvianolic acid A, a novel matrix metalloproteinase-9 inhibitor, reduces microvascular damage induced by hypertension

  • Research Article
  • Cite Count Icon 3
  • 10.1080/03115518.2016.1177290
First appearance datum of the Silurian graptolite Oktavites spiralis, and its evolution on the northern margin of South China
  • May 18, 2016
  • Alcheringa: An Australasian Journal of Palaeontology
  • Xin Wang + 2 more

Wang, X., Wang, J. & Zhang, J., May 2016. First appearance datum of the Silurian graptolite Oktavites spiralis, and its evolution on the northern margin of South China. Alcheringa 41, xxx–xxx. ISSN 0311-5518.Xin Wang [wx200315046@163.com], Early Life Institute and State Key Laboratory of Continental Dynamics, Department of Geology, Northwest University, Xian 710069, PR China & Research Center for Orogenic Geology, Xian Geological Survey Center, China Geological Survey, Xian 710054, PR China; Jian Wang [332891824@qq.com], Ju Zhang [710726657@qq.com], Research Center for Orogenic Geology, Xian Geological Survey Center, China Geological Survey, Xian 710054, PR China.Graptolites represent the standard tools for biozonation and correlation of Ordovician and Silurian strata. Oktavites spiralis, one of the index graptolite species of the Telychian Age (late Llandovery, Silurian), is of great significance in biostratigraphy. However, the broad definition of the species and the lack of data on its evolutionary changes have led to controversies regarding its stratigraphic range and, consequently, to difficulties in regional correlations. Recent investigations in multiple Telychian profiles in the Ziyang–Langao area, along the northern margin of South China, reveal that the first appearance datum of Oktavites spiralis is located at the base of the Spirograptus turriculatus Zone, and its last appearance datum lies at the top of the Cyrtograptus lapworthi Zone (Telychian stage). The thecae vary markedly up-section and such change is consistent across all studied sections. This morphological change can be used as the basis for accurate stratigraphic subdivision and, hence, for effective regional correlation. In addition, this species has a wide range in population size and population density relative to other coexisting graptolite species, and can be better distinguished by using a more precise set of criteria.

  • Research Article
  • Cite Count Icon 28
  • 10.1080/03115518.2015.1085749
New occurrence of Cambroclavus absonus from the lowermost Cambrian of North China and its stratigraphical importance
  • Oct 22, 2015
  • Alcheringa: An Australasian Journal of Palaeontology
  • Luoyang Li + 3 more

Li, L.-Y., Zhang, X.-L., Yun, H. & Li, G.-X., October 2015. New occurrence of Cambroclavus absonus from the lowermost Cambrian of North China and its stratigraphical importance. Alcheringa 40, xxx–xxx. ISSN 0311-5518.The problematic Small Shelly Fossil Cambroclavus absonus is described from the Xinji Formation in the Longxian area, which is located near the southwestern margin of the North China Platform. The Xinji Formation, the basal rock unit of the Cambrian in the studied area, yields an assemblage of skeletal fossils that share many common elements with contemporary faunas from South Australia. Sclerites of C. absonus reported herein represent the first occurrence of the species outside Australia, thus extending the palaeogeographic range of the taxon to northern China. To date, palaeogeographic occurrences of Cambroclavus sclerites are restricted to the Peri-Gondwana realm, including South China, Australia, Tarim, Kazakhstan, North China and Western Europe. These occurrences are divided into a Southern Group realm and Northern Group realm. Stratigraphically, Cambroclavus occurs mostly in Cambrian Stage 3 and has three occurrences in Stage 5, separated by Stage 4 in which Cambroclavus has not yet been found. The first appearance datum of Cambroclavus in Cambrian Stage 3 is of importance for regional and inter-regional correlations. In particular, the presence of Cambroclavus absonus in North China allows species-level correlation between North China and South Australia.Luoyang Li [lly@stumail.nwu.edu.cn], Xingliang Zhang [xzhang69@nwu.edu.cn], Hao Yun [yunhao@stumail.nwu.edu.cn], Early Life Institute and State Key Laboratory Of Continental Dynamics, Department of Geology, Northwest University, Xian 710069, PR China; Guoxiang Li [gxli@nigpas.ac.cn], Nanjing Institute of Geology and Palaeontology, Chinese Academy of Sciences, Nanjing 210008, PR China.

  • Research Article
  • Cite Count Icon 1
  • 10.3724/sp.j.1118.2018.17173
Reproductive biology of Syngnathus schlegeli on the coast of Zhaitang Island
  • Jan 1, 2018
  • Journal of Fishery Sciences of China
  • Yuxi Huang + 2 more

PDF HTML阅读 XML下载 导出引用 引用提醒 青岛斋堂岛附近海域薛氏海龙繁殖生物学 DOI: 作者: 作者单位: 中国海洋大学 教育部海水养殖重点实验室, 山东 青岛 266003 作者简介: 黄玉喜(1992-),男,硕士研究生,研究方向为资源增殖学.E-mail:huangyuxi92@163.com 通讯作者: 中图分类号: S917 基金项目: 海洋公益性行业科研专项(201405010). Reproductive biology of Syngnathus schlegeli on the coast of Zhaitang Island Author: Affiliation: Key Laboratory of Mariculture, Ministry of Education;Ocean University of China, Qingdao 266003, China Fund Project: 摘要 | 图/表 | 访问统计 | 参考文献 | 相似文献 | 引证文献 | 资源附件 | 文章评论 摘要:根据2015年3-5月在青岛斋堂岛附近海域使用张网采集的1250尾薛氏海龙()样本,分析了该水域薛氏海龙的性比、副性征、性腺指数、性腺成熟度与卵径、育儿袋中胚胎等繁殖生物学特征。结果表明,青岛斋堂岛附近海域薛氏海龙春季繁殖种群雌雄比例分别为63.28%和36.24%,未成年个体占0.48%,雌雄比为1.75:1,明显偏离1:1的性别比例;繁殖群体中雄性个体的性腺指数始终保持在较低水平,为0.01%~4.85%,雌性个体相对较高,为0.05%~128.99%,二者差异极显著(<0.01);组织学观察发现,性腺在3-5月份迅速发育成熟,雄性排精后精巢腔中仍剩余较多精细胞,表明雄性个体为多次排精;雌性Ⅲ~Ⅳ期卵巢中均分布有不同时相的卵子,且卵径分布呈现两个峰值,表明雌性个体为分批产卵;根据雌性个体V期卵巢中成熟卵子的卵粒数与雄性个体育儿袋中的平均怀卵数基本相同,育儿袋中胚胎的发育阶段也基本一致,推测其交配模式可能为一雄一雌制,抑或是雄性在同一天内与多个雌性交配的一雄多雌制。斋堂岛附近海域薛氏海龙的交配模式还有待室内行为观察并结合微卫星标记等手段进一步确认。 Abstract:Twelve hundred and fifty individuals of were sampled from the coast of Zhaitang Island, Qingdao (using stow nets, in 2015), and their sex ratio, secondary sexual characteristics, gonadosomatic index (GSI), gonad maturity levels, egg diameter, and number of embryos in the pouch were examined. The results showed that the abundance of females was higher than that of males in the area, with a total sex ratio of 1.75:1, deviating significantly from a 1:1 sex ratio, and the sample comprised 63.28% females, 36.24% males, and 0.48% underage individuals. The gonadosomatic index (GSI) range in males in the breeding population was maintained at a relatively low level of 0.01% to 4.85%, and the GSI in females ranged from 0.05% to 128.99%, and was significantly different from that of the males (S. schlegeli quickly matured from March to May. After spermiation, some sperm cells remained in the testis of male , showing that multiple spermiation events may occur. In the female breeding groups, different phases of eggs appeared in the stage Ⅲ-VI ovary, and the egg-diameter distribution had two peaks, indicating a multiple-batch spawning pattern. The number of mature eggs in the stage V ovary was in accordance with the number of eggs in the paternal brood bags, and the embryos in the paternal brood bags were at the same stage, suggesting monogamous mating, or polygamy whereby males mated with multiple females on the same day. Evidence for the mating patterns of on the coast of Zhaitang Island needs to be shown by behavioral observation and microsatellite analysis. 参考文献 相似文献 引证文献

  • Research Article
  • 10.3724/sp.j.1118.2018.17041
Estimates of genetic parameters for growth and shell color traits in the white-shell strain of Pacific oyster ( Crassostrea gigas )
  • Jan 1, 2018
  • Journal of Fishery Sciences of China
  • De Xing + 2 more

PDF HTML阅读 XML下载 导出引用 引用提醒 壳白长牡蛎品系生长和壳色性状遗传参数估计 DOI: 作者: 作者单位: 中国海洋大学, 海水养殖教育部重点实验室, 山东 青岛 266003 作者简介: 邢德(1992-),男,硕士研究生,从事贝类遗传育种研究.E-mail:xingzhidezhi@126.com 通讯作者: 中图分类号: S917 基金项目: 国家自然科学基金项目(31372524);泰山学者种业计划专家项目;山东省科技发展计划项目(2016ZDJS06A06). Estimates of genetic parameters for growth and shell color traits in the white-shell strain of Pacific oyster (Crassostrea gigas) Author: Affiliation: The Key Laboratory of Mariculture, Ministry of Education;Ocean University of China, Qingdao 266003, China Fund Project: 摘要 | 图/表 | 访问统计 | 参考文献 | 相似文献 | 引证文献 | 资源附件 | 文章评论 摘要:以经过连续4代家系选育获得的壳白长牡蛎()选育系为亲本,通过巢氏平衡设计建立了30个全同胞家系混合养殖,采用微卫星多重PCR技术进行家系鉴定,基于REML法估算24月龄壳白长牡蛎的生长性状和壳色性状的遗传参数。结果表明,壳白长牡蛎品系壳高、壳长、总重、壳重、(黄蓝轴色品指数)的遗传力为中高等水平,依次为0.35±0.13、0.18±0.09、0.20±0.09、0.16±0.08、0.16±0.08、0.27±0.11和0.19±0.08,壳宽、肉重、出肉率、壳型指数的遗传力为低等水平,依次是0.07±0.02、0.11±0.06、0.02±0.03、0.08±0.06和0.11±0.06。壳高、壳长、壳宽、总重、壳重和肉重之间的遗传相关和表型相关均为正相关,其中,壳高、壳宽和总重与其他生长性状的相关性较高,分别为0.40±0.65~0.90±0.14、0.39±0.55~0.97±0.24和0.50±0.66~0.99±0.02。壳型指数与壳高均为较高的负相关,分别为-0.94±0.16和-0.77±0.19,表明仅以壳高性状为选育目标时,可能不会对长牡蛎壳型改良产生作用。壳白长牡蛎壳色参数与生长性状之间的遗传相关范围为-0.09±0.42~0.91±0.74,不同性状间的遗传相关差异很大,其中*任一个为选育目标时,其他生长性状都可以获得提高。壳色参数间与相关性较低,分别为-0.08±0.36和0.21±0.31,表明以值。本研究为合理制定壳白长牡蛎新品系育种方案和选择反应预测提供了参考依据。 Abstract:The Pacific oyster () is the most widely cultured shellfish in the world. There is considerable focus on improving selective breeding procedures for economic traits. We used specimens of the white-shell strain of after four generations of family selection as parents to construct 30 full-sib families in a nested design. A mixed family approach combined with parentage analysis using highly polymorphic microsatellite makers and REML based on the animal model was used to estimate genetic parameters of white-shell at the age of 24 months. The results showed that heritabilities of shell height, shell length, total weight, shell weight, were 0.35±0.13, 0.18±0.09, 0.20±0.09, 0.16±0.08, 0.16±0.08, 0.27±0.11, and 0.19±0.08, respectively. Shell width, meat weight, meat ratio, and indexes of shell shape showed lower heritabilities of 0.07±0.02, 0.11±0.06, 0.02±0.03, 0.08±0.06, and 0.11±0.06, respectively. All phenotypic and genetic correlations among shell height, shell length, shell width, total weight, shell weight, and meat weight were positive. Genetic correlations were higher between shell height, shell length, total weight, and the other growth traits, ranging from 0.40±0.65 to 0.90±0.14, 0.39±0.55 to 0.97±0.24, and 0.50±0.66 to 0.99±0.02, respectively. Genetic correlation between index A of shell shape and shell height was -0.94±0.16, and -0.77±0.19 between index B of shell shape and shell height, suggesting that shell shape would not change if just shell height were selected in breeding programs. Genetic correlations were significantly different between shell color traits and growth traits, varying from -0.09±0.42 to 0.91±0.74. Higher genetic correlations between * (the same as shell height, shell length, and total weight) could improve other growth traits. There was a negative genetic correlation between of -0.96±0.04. Conversely, genetic correlations were lower between and could also decrease . This information will benefit genetic improvement of the white-shell strain of . 参考文献 相似文献 引证文献

  • Research Article
  • 10.3724/sp.j.1118.2018.17048
Effect of high temperature and hypoxia on median lethal time and physiological function in sea cucumber Apostichopus japonicus of two sizes
  • Jan 1, 2018
  • Journal of Fishery Sciences of China
  • Xiaomeng Zhou + 2 more

PDF HTML阅读 XML下载 导出引用 引用提醒 高温和低氧胁迫对两种规格刺参半致死时间及生理机能的影响 DOI: 作者: 作者单位: 1. 中国海洋大学, 海水养殖教育部重点实验室, 山东 青岛 266003;2. 青岛海洋科学与技术国家实验室, 海洋渔业科学与食物产出过程功能实验室, 山东 青岛 266071 作者简介: 周晓梦(1992-),女,硕士研究生,专业方向为渔业资源.E-mail:447189590@qq.com 通讯作者: 中图分类号: S96 基金项目: 国家海洋公益性行业科研专项(201405010);青岛海洋科学与技术国家实验室鳌山科技创新计划项目(2015ASKJ02). Effect of high temperature and hypoxia on median lethal time and physiological function in sea cucumber Apostichopus japonicus of two sizes Author: Affiliation: 1. Key Laboratory of Mariculture, Ministry of Education;Ocean University of China, Qingdao 266003, China;2. Functional Laboratory of Marine Fisheries Science and Food Production Process;Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China Fund Project: 摘要 | 图/表 | 访问统计 | 参考文献 | 相似文献 | 引证文献 | 资源附件 | 文章评论 摘要:通过实验生态学方法,针对2种规格的刺参()(大:28.00~36.00 g;小:9.00~13.00 g),测定温度为(25.0±0.5)℃溶解氧(DO)水平为(1.0±0.1)mg/L条件下刺参的半致死时间(LT50);比较分析了DO水平分别为(1.0±0.1)mg/L、(3.0±0.1)mg/L及6.5 mg/L(正常DO含量)条件下2种规格刺参的昼夜代谢水平;监测了DO水平分别为(1.0±0.1)mg/L和(3.0±0.1)mg/L时低氧胁迫24 h及复氧72 h期间,2种规格刺参体腔液和呼吸树中的谷胱甘肽(GSH)含量、总抗氧化能力(T-AOC)、超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性及体腔液中皮质醇水平的变化,以探究夏季高温期(25.0±0.5)℃低氧胁迫对不同规格刺参半致死时间及生理机能的影响。结果显示:当DO浓度为1 mg/L时,大规格刺参半致死时间(LT50)为33.37 h,小规格刺参为28.84 h,2种规格刺参的代谢水平显著低于常氧对照组,夜间代谢强度高于白天,且小规格刺参的代谢强度高于大规格刺参。1 mg/L低氧胁迫期间,大规格刺参体腔液及2种规格刺参呼吸树中上述4种抗氧化指标的变化趋势大致相同,与对照组相比,随着低氧暴露时间的延长,GSH含量下降,SOD和T-AOC活力降低,CAT活力升高;然而胁迫结束时其GSH含量、CAT、SOD和T-AOC活力均与对照组无显著差异(>0.05)。解除胁迫复氧72 h后,2种规格刺参体腔液的4种抗氧化指标均恢复到对照组水平;而呼吸树的这4种指标则未完全恢复。3 mg/L低氧胁迫期间,2种规格刺参体腔液和呼吸树的GSH含量、CAT、SOD和T-AOC活力的变化趋势与1 mg/L组大体一致,复氧72 h后体腔液的各项指标恢复到对照组水平;除T-AOC,呼吸树的其余3种指标亦完全恢复。低氧胁迫后小规格刺参的恢复能力高于大规格刺参。在DO为1 mg/L和3 mg/L条件下,胁迫结束时2种规格刺参体腔液中的皮质醇含量显著高于对照组(<0.05),且大规格刺参皮质醇含量高于小规格,复氧后二者均恢复到对照水平。结果表明,低氧胁迫持续时间不超过其半致死时间(本研究以24 h为例),刺参可通过自身调节减轻机体的氧化损伤;一旦超出半致死时间,将产生不可逆损伤,最终导致死亡。 Abstract:An experimental ecological method was used to determinate the effects of hypoxia on the median lethal time (LT50) and physiological function of two sizes of sea cucumber (large-size:with a body weight ranging from 28.00 g to 36.00 g; small-size:with a body weight ranging from 9.00 g to 13.00 g) at high temperature (25℃). The LT50 in the two sizes of sea cucumber at a dissolved oxygen (DO) concentration of 1 mg/L were investigated. The diurnal metabolism in the two sizes of sea cucumber at DO concentrations of 1, 3, and 6.5 mg/L (normoxia) were estimated. The glutathione (GSH) content and activities of superoxide dismutase (SOD), catalase (CAT), and total antioxidant capability (T-AOC) in the coelomic fluid and respiratory tree, as well as the cortisol level in the coelomic fluid at 24 h hypoxia stress (the DO concentrations of 1 mg/L and 3 mg/L) and 72 h reoxygenation of the two sizes of sea cucumber were also measured. The results showed that, at first, at the DO concentration of 1 mg/L, the LT50 for the large and small-size sea cucumber was 33.37 h and 28.84 h, respectively. The metabolism in the two sizes of sea cucumber was significantly lower than that in the normoxia condition (hereafter control), the overnight metabolic intensity was higher than during the daytime, and metabolic intensity in the small-size sea cucumber was higher than in the large-size one. Secondly, during the 1 mg/L hypoxia stress, the variations of the four antioxidant indices in the coelomic fluid in the large-size sea cucumber and those in the respiratory tree in both sizes of sea cucumber were similar:compared with the control group, the hypoxia exposure decreased the content of GSH and the activity of SOD and T-AOC, but increased the activity of CAT. However, at the end of the hypoxia stress, the content of GSH and the activity of SOD, CAT, and T-AOC showed no significant difference from the control group. After 72 h of reoxygenation, the above four antioxidant indexes of the coelomic fluid in the two size groups of sea cucumber recovered to the level of the control group but the four antioxidant indexes of the respiratory tree did not. During the 3 mg/L hypoxia, the variation trend of the four antioxidant indexes in the coelomic fluid and respiratory tree of sea cucumber was consistent with those in the 1 mg/L group. After 72 h of reoxygenation, the above four antioxidant indexes of the coelomic fluid in sea cucumber recovered to the level of the control group. Except for T-AOC, the other three indices of the respiratory tree also recovered. The recovery ability of the small-size sea cucumber was higher than that of the large one after hypoxia stress. Thirdly, at the DO concentrations of 1 mg/L and 3 mg/L, the content of cortisol in the coelomic fluid of both sizes of sea cucumber was significantly higher than the control group at the end of the stress (<0.05). The level of cortisol in the coelomic fluid of the large-size sea cucumber was higher than in the small one but both recovered to the control level after reoxygenation. The results showed that if the hypoxia duration is less than the LT50(24 h, for example, in this paper), sea cucumber can alleviate the oxidative damage. However, if the time under hypoxia exceeds the threshold value, sea cucumbers will suffer irreversible damage and die. 参考文献 相似文献 引证文献

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