Abstract
Fluorescence photobleaching recovery was used to measure the translational diffusion of bovine serum albumin (BSA) in DNA solutions as a function of DNA concentration (from 2 to 36 mg/mL) and added salt concentration (0.1 and 0.01 M NaCl). The DNA was 160 base pair mononucleosomal material, about 1 persistence length. Lowering the salt concentration decreases the BSA tracer diffusion coefficient, probably due to the expanded counterion atmosphere and consequently greater effective excluded volume of the polyions. The DNA-concentration dependence is fit well by an acessible volume fraction model and also by a stretched exponential function D/D 0 = exp(-ac ν ).
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