Abstract
The effect of leachate samples from Olushosun dumpsite in Ojota, Lagos on the toxicological response of Poecilia reticulata , Hyla and Culex species was investigated using acute and chronic toxicity tests. Physicochemical parameters were analysed in the leachate prior to toxicological assessment against test animals. Computed results showed that pH 7.62 while the acute toxicity assay of leachate sample against test animals revealed a 96hr LC 50 of 71.53ml/L, 58.34ml/L and 60.643ml/L for Poecilia, Hyla and Culex species respectively. On the basis of the 96hr LC 50 values, the leachate was found to be more toxic against Hyla than Poecilia . The exposure of the Poecilia and Hyla species to sub-lethal concentrations (1/10th of 96hrs LC 50 ) of leachate revealed varied biochemical responses over a 28 day period. The activity of Catalase, Superoxide Dismutase (SOD), Malondialdehyde (MDA) and Glutathione-S-Transferase (GST) showed significant increase (p<0.05) when compared to control in Hyla while the activity of GSH, SOD and CAT showed significant increase (p<0.05) when compared to control in Poecilia sp. Results from this study showed that leachate can induce adverse environmental effect on organisms. The acute and chronic toxicological response of the test animals could be incorporated into risk assessment of dumpsite leachate, design and management of landfill sites.
Highlights
Landfilling is the main municipal solid waste disposal method used worldwide (Dave and Nilsson, 2005)
Leachate from municipal landfill usually contain very low Dissolved Oxygen (DO) levels because of waste compression processes and due to aerobic decomposition of the wastes in which microbes use up oxygen to transform organic materials to inorganic substances (Bagchi, 1994)
Several bulk parameters are used to describe the content of dissolved organic matter in leachate, Total Organic Carbon (TOC), Chemical Oxygen Demand (COD) and Biological Oxygen Demand (BOD)
Summary
Landfilling is the main municipal solid waste disposal method used worldwide (Dave and Nilsson, 2005). Whereas high-molecular weight defences include enzymes such as superoxide dismutase (SOD), catalase, glutathione peroxidase (GPx), glutathione reductase (GRx) and glutathione-s-transferase (GST). These enzymatic and non-enzymatic constituents deal directly with radical species and protect against intoxication of any damage to the macro and micro molecules in body. The Ojota area of Lagos, Nigeria has the largest municipal landfill situated at Olushosun, it has received more than 50% of the total refuse in Lagos area since 1989. It receives domestic, industrial and institutional waste by public and private waste management operators. In the present study, the effect of leachate on the enzymes superoxide dismutase (SOD), Glutathione-S-Transferase (GST), reduced glutathione (GSH), Malondialdehyde (MDA) and catalase (CAT) activity in Poecilia reticulata and Hyla species were analyzed
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Journal of Applied Sciences and Environmental Management
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
