Abstract
Many studies have investigated the negative impacts of microplastics on teleost fishes with very little or no evidence of their mechanism of action. This scenario entreats us to investigate the toxicities of nanopolystyrene in zebrafish oocyte with emphasis on the mechanism of action. In the present study, the cellular levels of mRNA transcripts of different genetic markers (such as: sod, gpx, nrf2, inos, ucp2, and atp6 (redox-sensitive markers); nfkβ, tnfα, il-10, ikβ, gdf9, and bmp15 (immune markers); gadd45, rad51, p53 and bcl2 (DNA damage and apoptotic)) have been quantified by real-time PCR after 6 h of incubation of isolated oocyte with different doses of nanopolystyrene viz. P0 (control i.e. no polystyrene in culture medium), P1 (100 ng/ml), and P2 (400 ng/ml). Results showed that both the treatment concentrations of nanopolystyrene induce oxidative stress with % DPPH = 30.75, 31.61, and 32.43% for P0, P1, and P2, respectively. Increase in oxidative stress in oocytes with increasing doses of nanopolystyrene was also observed in TBARS assay with MDA content 0.12 and 0.21 μM for P1 and P2, respectively as compaired to the control 0.08 μM. This increased oxidative stress can regulate the expression pattern (upregulation/downregulation) of selected genes leading to different toxic effects like – oxidative stress, immunotoxicity, and apoptosis in oocytes, which suggests the impairment of reproductive functions by nanopolystyrene.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.