Abstract
Pseudomonas toxin inhibited protein synthesis in mouse LM fibroblast monolayers. Incubation of toxin with LM cell monolayers resulted in a depletion of functional elongation factor 2. The initial interaction of pseudomonas toxin with mouse LM cells was rapid; within 2.5 min, toxin was rendered inaccessible to neutralization with specific pseudomonas antitoxin. At 4°C toxin adsorbed to the cell surface, but remained at a site where it could be neutralized with antitoxin. Ammonium chloride (20 mM) rendered LM cells insensitive to the action of toxin. The ammonium salt did not prevent adsorption of toxin to the cell membrane; rather, it appeared to maintain toxin at a site amenable to antitoxin neutralization.
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