Abstract
Previously, our study reported that β-(1→3,1→6)-ᴅ-glucans produced by endophytes Diaporthe sp. G27-60 and G65-65 (GenBank accession codes JF766998 and JF767007, respectively) are promising antiproliferative agents against human breast carcinoma (MCF-7) and hepatocellular carcinoma (HepG2-C3A) cells. However, effects of Diaporthe exopolysaccharides on eukaryotic healthy cells are not described in current literature. The fungus Metarhizium anisopliae has been employed as teste-system to evaluate the toxicity of pharmaceutical and agricultural-interest substances, considering, among other parameters, conidia germination speed. The current study aimed to verify the effect of different concentrations of Diaporthe β-glucans on the germination speed of M. anisopliae. Conidia were incubated with β-glucans treatments (50, 200 and 400 μg/mL) at 28 ºC, sampled throughout 24 h and analyzed by light microscopy. At the end of 24 h of incubation, the amount of germinated conidia was ≈99% for controls and ranged from 97.7 to 98.6% for treatments. Bayesian analysis indicated that Diaporthe glucans had no toxicity on M. anisopliae and the curve of germination occurred as expected for this fungal strain. Considering the validity of filamentous fungi as model systems, these results are important data about the toxicity of these endophytic EPS on healthy cells and may be associated with previous results obtained for these polymers against tumor cells.
Highlights
The medicinal plant Piper hispidum Sw. harbors a diversity of endophytic fungi that inhabit the interior of the host plant without causing any damage (Orlandelli et al 2012a), which include strains that secrete compounds with antimicrobial and enzymatic activity (Orlandelli et al 2012b, 2015, 2017a)
In a previous paper (Orlandelli et al 2016), we identified that some strains are exopolysaccharide (EPS) producers: two of them, identified as Diaporthe (= Phomopsis) sp., secrete high-molecular weight EPS characterized as β(1→3,1→6)-ᴅ-glucans, with potent antiproliferation effects on tumor cells: inhibition ratios up to 74.6% and 83.3% against human breast carcinoma (MCF-7) and hepatocellular carcinoma (HepG2-C3A) cells, respectively (Orlandelli et al 2017b)
Current study is the first report on possible toxic effects of β-glucans from Diaporthe strains against eukaryotic healthy cells
Summary
The medicinal plant Piper hispidum Sw. (called cordoncillo in Mexico and falso-jaborandi in Brazil) harbors a diversity of endophytic fungi that inhabit the interior of the host plant without causing any damage (Orlandelli et al 2012a), which include strains that secrete compounds with antimicrobial and enzymatic activity (Orlandelli et al 2012b, 2015, 2017a). Different biological systems could be evaluated to provide an in-depth investigation on the toxic effects of these polymers on healthy cells. The germination speed of M. anisopliae conidia has been employed to evaluate the effects of nutritional and physical factors, and the toxicity or compatibility of pharmaceutical and agricultural-interest substances (Rangel, Alston and Roberts 2008, Alves et al 2011, Akbar et al 2012, Tonussi et al 2012, Bulla et al 2013, Fabrice et al 2013, Almeida et al 2014, Mochi et al 2017, Sohrabi et al 2019).
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
