Abstract
This study describes an investigation into the relationship among toxicity, specificity, and binding of CryIC δ-endotoxin fromBacillus thuringiensissubspeciesaizawaiHD-229.In vivobioassays of larvae using inclusions (Aedes aegypti) or solubilized toxin (Spodoptera littoralis, Spodoptera exigua, Spodoptera frugiperda,andManduca sexta) revealed that CryIC was toxic to both dipteran and lepidopteran larvae.In vitrosaturation binding assays with CryIC toxin labeledin vivowith [35S]methionine revealed specific and saturable binding to brush border membrane vesicles from both resistant and susceptible insect species. In contrast, dissociation binding experiments showed that the relative toxicities and specificities of CryIC to the insect species tested were correlated with the extent of irreversible binding to the insects' midgut brush border membrane vesicles.
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