Torque and Velocity Dependence of Muscle Fatigue in Aging

Abstract

Old age generally leads to smaller, weaker and slower skeletal muscles. To address the independent effects of weakness vs. slowing on fatigue in aging, we used a custom ergometer in a whole-body, 3 tesla magnetic resonance system to quantify knee extensor size, torque, velocity, power and intracellular energetics at baseline and during two 4-min fatiguing contraction protocols; one in which contraction velocity was constrained and torque varied (i.e., torque-dependent contractions; isokinetic, IsoK), and one in which torque was constrained and velocity varied (i.e., velocity-dependent contractions; isotonic, IsoT). On separate days, 10 young (27.5±1.2 yrs, 6 men) and 10 older (71.2±1.6 yrs, 5 men) healthy adults completed the IsoK (120°∙s-1, 0.5 Hz) and IsoT (20% maximal torque, 0.5 Hz) protocols, with continuous measures of intracellular [Pi], pH, and [H2PO4-]. At baseline, contractile volume (803.5±72.3 vs. 1,125.6±109.9cm3), specific IsoK torque (0.035±0.004 vs. 0.058±0.007Nm.cm-3) and IsoT velocity (121.4±11.6 vs. 176.3±8.0deg.s-1) were greater in young than older (p≤0.023). Fatigue (%initial specific torque) was greater in young than older for IsoK (40.1±3.0 vs. 61.2±5.3%, p=0.0028), and accompanied by greater [Pi] and [H2PO4-] and lower pH in the young (p≤0.001). For IsoT, fatigue (%initial velocity) was not different between groups (young: 56.5±5.5 vs. older: 47.2±4.9%, p=0.661), despite lower pH and greater [H2PO4-] in young than old (p≤0.001). Collectively, these results reveal that normalizing dynamometer outputs to assess age-related differences in fatigue obscures baseline differences in muscle weakness. Further, our results suggest the contractile machinery may be less sensitive to changes in pH in older than young.